Marathon-Ready cDNA—made from high-quality poly A+ RNA and ligated to the Marathon Adaptor—is ready for 5'- and 3'-RACE PCR (Chenchik et al. 1996). Marathon-Ready cDNA is a tissue-specific "pool" of premade double-stranded cDNA from which full-length transcript sequences can be amplified using gene-specific primers. It can also be used to study tissue-specific gene expression and to find polymorphic forms of mRNA, or mRNA belonging to a multigene family.
This Marathon-Ready cDNA is synthesized from premium RNA derived from various human fetal tissuesusing a procedure optimized to produce full-length cDNA and to eliminate 3' heterogeneity (Borson, Salo, and Drewes 1992). After synthesis, blunt ends are created and the Marathon Adaptor is ligated to both ends of the double-stranded cDNA.
Adaptor-ligated and ready for RACE PCR
Tissue-specific "pool" of double-stranded cDNA
Obtain full-length cDNAs without ever constructing or screening a cDNA library
Perfect template for Marathon cDNA Amplification
Rapid amplification of cDNA ends (RACE)
Study tissue-specific gene expression
Discover polymorphic forms of mRNA
Obtain full-length copies of published cDNAs
Characterization of tissue-specific patterns of gene expression
For current source information, please refer to the Certificate of Analysis accompanying each Marathon-Ready cDNA.
Borson, N. D., Salo, W. L. & Drewes, L. R. A lock-docking oligo(dT) primer for 5' and 3' RACE PCR. PCR Methods Appl.2, 144-8 (1992).
Chenchik, A. et al. Full-length cDNA cloning and determination of mRNA 5' and 3' ends by amplification of adaptor-ligated cDNA. Biotechniques21, 526-34 (1996).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.