High-throughput single-cell isolation, selection, and processing with the SMARTer ICELL8 Single-Cell System
The SMARTer ICELL8 Single-Cell System is an advanced automation platform that combines the power of imaging with the isolation of single cells in 5,184 nanowells. In contrast with other platforms, the SMARTer ICELL8 system employs a large-bore nozzle dispenser for unbiased cell size isolation. Imaging allows the differentiation of single cells from multiple cells.
The SMARTer ICELL8 Single-Cell System is an advanced automation platform that combines the power of imaging with the isolation of single cells in 5,184 nanowells. In contrast with other platforms, the SMARTer ICELL8 system employs a large-bore nozzle dispenser for unbiased cell size isolation. Imaging allows the differentiation of single cells from multiple cells. Additionally, with the use of fluorescence, imaging can also be used for live/dead analysis in conjunction with the use of cellular dyes. The system comes with integrated CellSelect Software that analyzes the images and provides the ability to proceed only chosen wells, eliminating the noise from multiple-cell-containing wells or empty wells. The SMARTer ICELL8 Single-Cell System has a modular design with the ICELL8 Imaging System and ICELL8 MultiSample Dispenser provided as separate units.
The SMARTer ICELL8 platform allows parallel processing of up to eight samples, making it suitable for performing replicates and eliminating batch effects. While the system comes validated with various applications, it is an open platform that allows the user to develop their own applications of choice.
- Automated dispensing of cells and reagents by the ICELL8 MultiSample NanoDispenser
- Accommodates up to eight samples
- Flexible options for volume and pattern dispensation
- Unbiased isolation of 1,000–1,800 single cells in a 5,184-nanowell chip
- Automated rapid and robust imaging with the ICELL8 Imaging System
- Simple user interface
- Imaging channels for live/dead analysis of cells*
*CellTracker Red or a combination of Hoechst and propidium iodide dyes can be used for cell identification and viability assessment.
- Autoselect single cells or nuclei for downstream analysis with CellSelect software
- Evaluate staining for cell viability
- Distinguish single-cell-containing wells from empty and doublet-containing wells
Chips and reagents (sold separately)
- 5,184-nanowell chips
- Chips of varying depth hold 150, 250, or 350 nl
- Blank chips and preprinted chips for specific applications
- Differential expression by 3' end counting
- TCR profiling
- Full-length transcriptome analysis
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Available SMARTer ICELL8 applications
The SMARTer ICELL8 systems give researchers the flexibility to choose from prevalidated SMARTer NGS chemistries or develop custom applications. Adaptations of our SMART-Seq v4 technology for library preparation on the SMARTer ICELL8 systems enable fast, reliable, high-throughput cDNA synthesis for downstream NGS applications. This pairing provides results with high similarity to those of manually generated libraries while increasing reproducibility.Learn more
SMARTer ICELL8 single-cell analysis
Products formerly sold by WaferGen Bio-Systems are now sold by Takara Bio. If you have a WaferGen catalog number that you have previously used for ordering, please use the corresponding Takara Bio catalog number to place an order.
|Takara Bio Cat. #||WaferGen Cat. #||Product|
|640000||420-000043||SMARTer ICELL8 Single-Cell System|
|640018||430-000025||MSND 384-Well Source Plate and Seals (20 Pack)|
|640037||430-000258||MSND 384-Well Source Plate and Seals (120 Pack)|
|640146||430-000248||SMARTer ICELL8 Blank Chip Kit|
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