SMARTer PrepX reagents for simple, automated NGS library prep
SMARTer PrepX kits provide every reagent necessary for Illumina sequencing library prep in prealiquoted, color-coded, single-use reagent strips—making SMARTer Apollo deck setup effortless. SMARTer PrepX kits and scripts are designed with flexibility in mind to accommodate a variety of sample throughputs. Setup is simple: follow the prompts on the graphic user interface that tell you exactly where and how to place the reagents, minimizing error and stress. Just set up the deck, hit Run, and walk away.
- Prealiquoted, color-coded, single-use reagent strips
- Generate reproducible, high-quality NGS libraries, regardless of input concentrations, within a defined range
- Accommodate a variety of sample throughputs
- Seamless solution for consistent DNA-seq, RNA-seq, and ChIP-seq NGS library prep
- Eliminates the extensive hands-on and labor-intensive workflows required by many protocols
Sample input amount and quality are important considerations for any sequencing application. Committing too much of your sample to a workflow or getting a low yield means wasted material, delayed experiments, poor results, or insufficient downstream sequencing material. SMARTer PrepX kits and related scripts resolve these issues by generating comparable, high-quality NGS libraries regardless of input concentrations within a defined range (Table I).
|Table I. Reliable sequencing coverage and performance.
|Input amount||10 ng||100 ng|
|Adapter reads (%)||0||0|
|Confidently mapped reads (%)||96.61||96.43|
|Repetitive reads (%)||1.84||1.80|
|Low-quality reads (%)||0||0|
For the experiment above, high-quality libraries were prepared using the SMARTer PrepX DNA library prep kit on the SMARTer Apollo system from either 10 ng or 100 ng of E. coli genomic DNA. Libraries were sequenced on the Illumina MiSeq® platform (2 x 150 bp).
Each SMARTer PrepX chemistry is compatible with a script with variable throughputs and minimum input requirements, as summarized in Table II.
|Table II. Input amounts required for various combinations of SMARTer PrepX kits and preprogrammed SMARTer Apollo scripts.|
|Cat. #||SMARTer Apollo reagent kit||Compatible script||Script throughput||Minimum input amount per sample|
|640161*||SMARTer PrepX ILMN DNA Library Kit - A||PrepX ILM 8||1–8||1 ng DNA|
|640162*||SMARTer PrepX ILMN DNA Library Kit - B||PrepX ILM 32i||4–32||1 ng DNA|
|640096||SMARTer PrepX RNA-Seq for Illumina Library Kit, 24 Samples||PrepX mRNA 8||1–8||100 pg poly A-enriched RNA; 2 ng rRNA-depleted mRNA|
|640097||SMARTer PrepX RNA-Seq for Illumina Library Kit, 48 Samples||PrepX mRNA 48||6–48||100 pg poly A-enriched RNA; 2 ng rRNA-depleted mRNA|
|640098||SMARTer PrepX PolyA mRNA Isolation Kit, 96 Samples||PrepX PolyA 8 or PrepX PolyA 48||1–8 or 6–48||100 ng total RNA|
|640099||SMARTer PrepX Small RNA for Illumina Library Kit, 24 Samples||PrepX small RNA 8||1–8||100 ng total RNA|
|640100||SMARTer PrepX Small RNA for Illumina Library Kit, 48 Samples||PrepX small RNA 48||6–48||100 ng total RNA|
*Cat. # 640161 provides enough reagent to run 24 samples in batches of up to 8 samples. Cat. # 640162 provides enough reagent to run 96 samples in batches of up to 32 samples.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
SMARTer Apollo NGS library preparation
Products formerly sold by WaferGen Bio-Systems are now sold by Takara Bio. If you have a WaferGen catalog number that you have previously used for ordering, please use the corresponding Takara Bio catalog number to place an order.
|Takara Bio Cat. #||WaferGen Cat. #||Product|
|640078||100003||SMARTer Apollo library prep system|
|640101||400075||PrepX Complete ILMN DNA Library Kit, 24 Samples|
|640102||400076||PrepX Complete ILMN 32i DNA Library Kit, 96 Samples|
|640096||400039||PrepX RNA-Seq for Illumina Library Kit, 24 Samples|
|640097||400046||PrepX RNA-Seq for Illumina Library Kit, 48 Samples|
|640098||400047||PrepX PolyA mRNA Isolation Kit, 96 Samples|
|640099||400073||PrepX Small RNA for Illumina Library Kit, 24 Samples|
|640100||400074||PrepX Small RNA for Illumina Library Kit, 48 Samples|
|640082||300019||SMARTer Apollo 0.2 ml PCR 8-Tube Strips, Clear|
|640086||300029||SMARTer Apollo Caps for 0.2 ml PCR 8-Tube Strips, Clear|
|640088||300033||SMARTer Apollo 1.1 mL MiniTubes|
|640083||300026||SMARTer Apollo Microtiter Plates|
|640084||300027||SMARTer Apollo Filter Tips|
|640085||300028||SMARTer Apollo Piercing Tips|
|640089||500005||SMARTer Apollo 96 Tip Rack|
|640087||300031||SMARTer Apollo Reservoirs|
|640091||500007||SMARTer Apollo Stylus Pen|
|640090||500006||SMARTer Apollo Stylus Pen Holder|
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