The ICELL8 system allows for a high-throughput, nanowells-based method to isolate nuclei or single cells of any size—and the flexibility to analyze multiple parameters in single experiments. It also uses the power of imaging to distinguish true single cells from multiple cells and live cells from dead cells—allowing you to draw meaningful conclusions from the data.
But as automation instruments become more time and cost efficient in the research field, they produce thousands of data points to analyze per run. Software that can take that information and transform it into a format for the scientist to more readily use is vital.
The hanta™ R kit is bioinformatic software for user-friendly analysis of sequencing data derived from Takara Bio platforms. The mappa Analysis Pipeline can be used to prepare files ready for import into hanta software.
Key update in version 2.0: more choices for Principal Component Analysis
Beginning with hanta version 2.0, top Principal Components can be reduced and visualized using the Uniform Manifold Approximation and Projection (UMAP, recommended) method as well as the t-Distributed Stochastic Neighbor Embedding (tSNE) method.
Upon acceptance of the EULA and submitting the form, you will be sent an email with a key required to install hanta Software. Follow the installation instructions in the hanta R Kit User Guide. hanta Software includes the following files:
- hanta GUI launcher (—check to see if this works on all OSes or just Windows or Mac)
- Command-line interface program (Windows, MacOS, or Linux)
- Example data files—can be used to test the Analyzer functionality (hanta Software data object (*.rda), gene_info.csv, gm.csv, and metadata.csv)
- Sample output—compare to the analysis results of the example data (—check to see if sample output is included--docs don't mention it)
Supported operating systems
- Windows 7
- macOS (Sierra v10.12.6)
- Linux CentOS (v6.9+)
- 8 GB RAM
Additional third-party software dependencies*
- RStudio Desktop
- Pandoc (optional; only required for advanced users that wish to forgo RStudio and run R from the command line)
*Not included with the software; must be downloaded and installed separately.
Additional product information
For use in conjunction with workflows on the ICELL8 cx Single-Cell or ICELL8 Single-Cell System System. More information is available in the hanta Software (version 2.0) User Guide. (KW note: right now this link points to the 1.0 software. Either it will be updated or 1.0 and 2.0 will sit side by side. Need clarification on this. Changes need to be consistently applied everywhere including Marketo form, CEC x104007, confirmation page, etc.)
hanta Software version history
|Version number||Release date||Notes|
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