Anti-human RIG-I antibody
Retinoic acid-inducible gene I protein (RIG-I) is double-stranded (ds) RNA helicase that is encoded by the DDX58 gene in humans. RIG-I is a member of the DEAD box protein family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). In addition to the RNA helicase domain, RIG-I contains a caspase recruitment domain. RIG-I binds to 5'-triphosphorylated single-stranded (ss) RNA and short dsRNA (<1 kb). It is involved in recognition of viral dsRNA and regulation of the immune response to viral infection. RIG-I has also been suggested to play roles in granulocyte production and differentiation, bacterial phagocytosis, and regulation of cell migration.
This product is an affinity-purified IgG antibody that recognizes human RIG-I protein. The antibody was raised in rabbit using a synthetic peptide and can be used for Western blot (WB) or immunocytochemistry detection of human RIG-I protein.
- Target Name: Human RIG-I protein
- Antigen: Human RIG-I CTD domain synthetic peptide
- Specificity: Cross-reacts with mouse RIG-I
- Application: WB, ICC
- Target Entrez Gene ID: 23586
Recommended antibody concentration (the optimal concentration should be determined empirically):
- Western blot: 1 µg/ml
- Immunocytochemistry: 1 µg/ml
Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.
Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 ml H2O (final concentration 100 µg/ml).
DDX58, retinoic acid-inducible gene I protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58, DEAD box protein 58, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, probable ATP-dependent RNA helicase DDX58, RIG1, RIG-I-like receptor 1 (RLR-1), DKFZp434J1111, FLJ13599
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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