Human Ago2 N-terminal monoclonal antibody
Human Ago2 N-terminal monoclonal antibody, also known as Argonaute 2 antibody, is generated against the N-terminal region of human recombinant protein Argonaute 2, a member of the Argonaute family of proteins. Argonaute 2 is a transcription factor that is required for RNA-mediated gene silencing or RNA interference (RNAi). Argonaute proteins bind small interfering RNA (siRNA) and exhibit endonuclease activity against RNA-RNA duplexes consisting of mRNA bound to its complementary siRNA fragment. This Ago2 antibody is suitable for use in Western blot analysis.
- Western blot analysis under reducing and non-reducing conditions
Human Ago2 N-terminal monoclonal antibody was obtained by fusing the mouse myeloma cell line P3U1 with lymph cells of BALB/c mouse lymph cells following immunization with the N-terminal peptide (1–148) of recombinant human Argonaute 2 (Ago2) protein (NP_036286.2). The Argonaute 2 antibody was purified from mouse ascitic fluid.
This product does not contain preservatives.
The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for up to 1 year or at 4°C for up to 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw. Diluted antibody should not be stored.
|hAgo2 1B1||Human Ago2 protein||Mouse IgG2a (lambda)|
Dissolve the lyophilized antibody in 100 μl of distilled water (final concentration: 2.0 mg/ml). This solution can be used as a stock solution. If further dilution is required, use the noted dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μl or more of the noted dilution solution.
Note (1): Be sure to store the antibody at a minimum concentration of 2.0 mg/ml. A lower antibody concentration may result in decreased stability.
Note (2): Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
10 mM PBS (pH7.4)
* When stored at 4°C, 0.1% sodium azide should be added as a preservative.
Azuma-Mukai, A. et al. Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing. Proc. Natl. Acad. Sci. U. S. A. 105, 7964-9 (2008).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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