Calpastatin (CAST) is a calpain inhibitor, a calcium-dependent cysteine protease that is widely distributed in higher-order animals. There are different types of calpastatins; examples include the 68-kDa erythrocyte-derived calpastatin and the 107-kDa myocyte-derived calpastatin (as evidenced by SDS-PAGE). Through its inhibition of calpain, calpastatin is believed to play important roles in the regulation of cell proliferation, differentiation, and aging. CAST is also involved in the proteolysis of amyloid precursor protein (APP).
Human Calpastatin Antibody (Monoclonal) (CSL1-5 clone) is raised against recombinant myocyte-derived calpastatin.
- Sensitive and specific measurement of calpastatin levels in cell culture supernatant
- Western blot analysis under reducing and non-reducing conditions
|Clone||Cat.#||Immunogen||Immunized host||Subclass||Antibody titer|
|CSL1-5||M045||Human calpastatin||BALB/c mouse||IgG1||1:4,000|
Stock solution (2.0 mg/ml) aliquots should be stored at –20°C for up to 1 year, or at 4°C for up to 6 months.
This antibody specifically reacts with human calpastatin.
Dissolve lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml).
Asada, K. et al. cDNA cloning of human calpastatin: sequence homology among human, pig, and rabbit calpastatins. J. Enzyme Inhib. 3, 49–56 (1989).
Engvall, E. & Perlmann, P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J. Immunol. 109, 129–35 (1972).
Towbin, H., Staehelin, T. & Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U. S. A. 76, 4350–4 (1979).
Uemori, T. et al. Characterization of a functional domain of human calpastatin. Biochem. Biophys. Res. Commun. 166, 1485–93 (1990).
Yokota, H. et al. Direct measurement of calpastatin subtypes by sandwich enzyme immunoassay using monoclonal antibodies. Mol. Cell. Probes 5, 261–9 (1991).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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