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Calpastatin detection

Calpastatin (CAST) is a calpain inhibitor, a calcium-dependent cysteine protease that is widely distributed in higher-order animals. There are different types of calpastatins; examples include the 68-kDa erythrocyte-derived calpastatin and the 107-kDa myocyte-derived calpastatin (as evidenced by SDS-PAGE). Through its inhibition of calpain, calpastatin is believed to play important roles in the regulation of cell proliferation, differentiation, and aging. CAST is also involved in the proteolysis of amyloid precursor protein (APP).

Human Calpastatin Antibody (Monoclonal) (CSL1-5 clone) is raised against recombinant myocyte-derived calpastatin.

Cat. #	Product	Size		License	Quantity	Details
M045	Monoclonal Anti-Human Calpastatin (Clone CSL1-5)	0.1 mg	USD $457.00
This monoclonal anti-human calpastatin antibody (CSL1-5 clone) was raised against recombinant muscle-type calpastatin. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Resources Back Recognition Sites of the Anti-Human Calpastatin Antibodies Recognition Sites of the Anti-Human Calpastatin Antibodies.

More Information

Applications

Sensitive and specific measurement of calpastatin levels in cell culture supernatant
Western blot analysis under reducing and non-reducing conditions

Source

Clone	Cat.#	Immunogen	Immunized host	Subclass	Antibody titer
CSL1-5	M045	Human calpastatin	BALB/c mouse	IgG₁	1:4,000

Specificity

This antibody specifically reacts with human calpastatin.

Form

Lyophilized. Dissolve lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml).

Product citations

Asada, K. et al. cDNA cloning of human calpastatin: sequence homology among human, pig, and rabbit calpastatins. J. Enzyme Inhib. 3, 49–56 (1989).

Engvall, E. & Perlmann, P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J. Immunol. 109, 129–35 (1972).

Towbin, H., Staehelin, T. & Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U. S. A. 76, 4350–4 (1979).

Uemori, T. et al. Characterization of a functional domain of human calpastatin. Biochem. Biophys. Res. Commun. 166, 1485–93 (1990).

Yokota, H. et al. Direct measurement of calpastatin subtypes by sandwich enzyme immunoassay using monoclonal antibodies. Mol. Cell. Probes 5, 261–9 (1991).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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