Stem cell factor detection
Stem cell factor (SCF) and its receptor c-Kit can exist both as transmembrane proteins and as soluble proteins. SCF is also known as Kit ligand (KL-1, Kitl), steel factor (SF), mast cell growth factor (MGF), familial progressive hyperpigmentation 2 (FPH2), and SHEP7. It plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. Functional SCF and c-Kit are critical to the development and survival of hematopoietic stem cells. Interaction of SCF with c-Kit, a tyrosine kinase, results in increased autophosphorylation activity.
This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human SCF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble human SCF protein in cell culture supernatant.
- Capable of measuring human SCF in cell culture supernatant
- Sensitivity: 7.14 pg/ml
|Precoated plate: Anti-Human SCF (K089) Rabbit IgG Affinity-Purified||96 well x 1|
|Labeled target: HRP conjugated Anti-Human SCF (K151) Rabbit IgG Fab Affinity-Purified||0.4 ml x 1|
|Standard: Recombinant Human SCF||0.5 ml x 2|
|EIA buffer: 1% BSA, 0.05% Tween 20 in PBS||30 ml x 1|
|Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS||12 ml x 1|
|Chromogen: TMB solution||15 ml x 1|
|Stop solution: 1N H2SO4||12 ml x 1|
|Wash buffer (40X): 0.05% Tween 20 in phosphate buffer||50 ml x 1|
- Range of assay: 50–3,200 pg/ml
- Assay sensitivity: 7.14 pg/ml
- Assay duration: Approximately 3–3.5 hr
- Specificity: Human SCF
- Species cross-reactivity: n/a
- Test specimen type: cell culture supernatant
- Sample volume per well: 100 µl
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2018 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.