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Home › Products › Antibodies and ELISA › Primary antibodies and ELISAs by research area › Cancer and inflammation › Hepatocyte growth factor

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Hepatocyte growth factor detection

Hepatocyte growth factor (HGF) belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. HGF is secreted by mesenchymal cells as a single inactive polypeptide that is cleaved by a serine protease (known as hepatocyte growth factor activator, or HGFA) into a 69-kDa alpha chain and 34-kDa beta chain. The active form of HGF is a heterodimer, produced by disulfide bond formation between the alpha and beta chains. HGF acts as a multifunctional cytokine in epithelial cells. It regulates cell growth, motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. HGF plays a role in tissue regeneration, angiogenesis, and tumorigenesis.

Hepatocyte growth factor (HGF) belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. HGF is secreted by mesenchymal cells as a single inactive polypeptide that is cleaved by a serine protease (known as hepatocyte growth factor activator, or HGFA) into a 69-kDa alpha chain and 34-kDa beta chain. The active form of HGF is a heterodimer, produced by disulfide bond formation between the alpha and beta chains. HGF acts as a multifunctional cytokine in epithelial cells. It regulates cell growth, motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. HGF plays a role in tissue regeneration, angiogenesis, and tumorigenesis.

ELISAs for hepatocyte growth factor detection

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human total HGF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human total HGF protein in serum, EDTA plasma, or cell culture supernatants.

Antibodies for hepatocyte growth factor detection

These products are affinity-purified IgG antibodies that recognize human or rat HGF protein (alpha chain). The antibodies were raised in rabbit using synthetic peptides and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection.

Alternative names

HGF is also known as hepapoietin A, hepatopoeitin A, scatter factor (SF), fibroblast-derived tumor cytotoxic factor (F-TCF), HGFB, HPTA, and deafness, autosomal recessive 39 (DFNB39).

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Cat. # Product Size Price License Quantity Details
18135A Anti-Human HGF alpha(H487) Rabbit IgG Affinity Purify 10 ug $125.00

Hepatocyte Growth factor (HGF) was discovered as a mitogen for hepatocytes. HGF was subsequently found to be identical to the scatter factor, which destroys epithelial cell adhesion and promotes cell movement. Some reports have shown that HGF is expressed in normal and malignant mammary epithelium. HGF has also been reported to promote motility and growth of epithelial cells, to induce morphogenesis of epithelial cells, and to promote vascularization. It has been speculated that HGF is involved in the growth and metastasis of cancer cells. The first step in the initiation of HGF action is dependent on its biding to a specific cell surface receptor, the HGF receptor, encoded by the proto-oncogene c-Met. It has been suggested that c-Met mediates both responses, i.e., promotion of growth and motility of HGF. HGF is synthesized as a 728 amino acid polypeptide that is processed to generate the mature growth factor consisting of a disulfide-linked 69-kDa α chain and a 34-kDa β chain.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents

Overview

Antigen Cat. # Application Species (Clone) and source Cross‑reactivity
Total HGF 27402A ELISA Human Capture:
(n/a)
Rabbit IgG

Detect: HRP (n/a)
Goat IgG
≤0.1% cross‑reactivity with human HAI-1, HAI-2, c-Met, and HGFA
HGF alpha (N‑terminus) 18281A,B IHC, WB Rat (H56)
Rabbit IgG
Does not cross-react with rat HGF beta chain or human HGF alpha/beta
HGF alpha (N‑terminus) 18131A,B IHC, WB Human (H55)
Rabbit IgG
Reacts with rat HGF alpha

Does not cross-react with human HGF beta
HGF beta (C‑terminus) 18132A,B IHC, WB Human (H714)
Rabbit IgG
Does not cross-react with rat HGF
HGF alpha 18133A IHC, WB Human (H163)
Rabbit IgG
Cross-reacts with rat HGF alpha

Does not cross-react with human HGF beta
HGF beta (N‑terminus) 18134A,B IHC, WB Human (H495)
Rabbit IgG
Cross-reacts with rat HGF beta

Does not cross-react with human HGF alpha
HGF alpha (C‑terminus) 18135A,B IHC, WB Human (H487)
Rabbit IgG
Cross-reacts with rat HGF alpha

Does not cross-react with human HGF beta

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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