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Neuron-specific enolase (NSE) detection
Three enolase isozymes are known in mammals, each with a characteristic tissue distribution: alpha, beta, and gamma enolase. The ENO2 gene encodes enolase 2 (gamma, neuronal) and is expressed in mature neurons and cells of neuronal origin. It is also known as neural enolase, neuron-specific enolase (NSE), 2-phospho-D-glycerate hydrolyase, gamma-enolase, neuron-specific gamma enolase, and enolase 2. NSE exhibits neurotrophic and neuroprotective properties in central nervous system (CNS) neurons. The calcium-regulated binding of NSE to cultured neocortical neurons promotes cell survival. NSE was detected in small-cell carcinomas of neuroendocrine origin, and has been proposed as a marker for lung cancer patients.
This product is an affinity-purified IgG antibody that recognizes NSE protein. The antibody was raised in mouse using NSE protein and can be used for Western blot (WB) detection and immunohistochemical (IHC) detection of NSE protein.
- Target Name: NSE protein
- Antigen: NSE protein (gamma-gamma homodimer)
- Application: WB, IHC
- Target Entrez Gene ID: 2026
Recommended antibody concentration (the optimal concentration should be determined empirically):
- Western blot: 200X dilution
- Immunohistochemical techniques: 200X dilution
Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.
Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 ml H2O (final concentration 250 µg/ml).
enolase 2 (gamma, neuronal); ENO2, neural enolase, neuron-specific enolase, NSE, 2-phospho-D-glycerate hydrolyase, gamma-enolase, neuron-specific gamma enolase, enolase 2
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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