- Alzheimer's disease
- Bone research
- Brain and CNS
- Cancer and inflammation
- Cell adhesion and ECM
- Environmental hazards
- Epigenetic antibodies
- Metabolic diseases
- mTOR signaling
- Signal transduction
- Stem cell research antibodies
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- Miscellaneous research areas
Neurofilaments (NF) are a key component of the neuronal cytoskeleton, and are responsible for providing structural support to the axon and regulating axon diameter. Mammalian neurofilaments are composed of three major subunits, classified based on molecular weight in SDS-PAGE: light (NF-L), 68–70 kDa; medium (NF-M), 145–160 kDa; and heavy (NF-H), 200–220 kDa. In humans, these proteins are encoded by the NEFL, NEFM, and NEFH genes, respectively. NF-M is also known as neurofilament, medium polypeptide; neurofilament, medium polypeptide 160kDa; NEF3, neurofilament 3, neurofilament triplet M protein, and 160 kDa neurofilament protein. NF-H is also known as neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; KIAA0845, neurofilament triplet H protein, and 200 kDa neurofilament protein.
This product is an affinity-purified IgG antibody that recognizes human and bovine NF-M and NF-H subunits. The antibody was raised in mouse using bovine brain neurofilament and can be used for immunohistochemical (IHC) detection of human and bovine NF-M and NF-H subunits.
- Target Name: Bovine NF-M and NF-H subunits
- Antigen: Bovine brain neurofilament protein
- Specificity: Reacts with bovine and human NF-M and NF-H proteins
- Application: IHC
- Target Entrez Gene ID: 281347, 528842
Recommended antibody concentration (the optimal concentration should be determined empirically):
- Immunohistochemical techniques: 200X dilution
Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.
Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 0.5 ml H2O.
NF-M: Neurofilament, medium polypeptide; neurofilament, medium polypeptide 160kDa; NEF3, neurofilament 3, neurofilament triplet M protein, 160 kDa neurofilament protein NF-H: neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; KIAA0845, neurofilament triplet H protein, 200 kDa neurofilament protein.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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