- Alzheimer's disease
- Bone research
- Brain and CNS
- Cancer and inflammation
- Cell adhesion and ECM
- Environmental hazards
- Epigenetic antibodies
- Metabolic diseases
- mTOR signaling
- Signal transduction
- Stem cell research antibodies
- Virus research
- Miscellaneous research areas
Nestin is a member of the intermediate filament (IF) protein family and is expressed primarily in neuronal precursor cells. Upon differentiation, nestin gene expression is downregulated and it is replaced by tissue-specific IF proteins. Nestin expression has been used as a marker for central nervous system (CNS) progenitor cells. Recent studies have indicated that it is also expressed in follicle stem cells and proliferating endothelial cells. Nestin is also known as FLJ21841 and Nbla00170.
This product is an affinity-purified IgG antibody that recognizes human nestin protein. The antibody was raised in rabbit using a synthetic peptide, and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human nestin protein.
- Target Name: Human nestin protein
- Antigen: Human nestin C-terminal synthetic peptide
- Specificity: Cross-reacts with rat and mouse nestin
- Application: WB, IHC
- Target Entrez Gene ID: 10763
Recommended antibody concentration (the optimal concentration should be determined empirically):
- Western blot: 1–5 µg/ml
- Immunoprecipitation: 5 µg/ml
Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.
Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 ml H2O (final concentration 100 µg/ml).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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