- Alzheimer's disease
- Bone specific alkaline phosphatase
- Collagen type 2
- Dentin matrix protein 1
- Osteocalcin (bovine)
- Osteocalcin (human)
- Osteocalcin (mouse)
- Osteocalcin (pig)
- Osteocalcin (rat)
- Osteocalcin carboxylated Gla-OC
- Osteocalcin undercarboxylated Glu-OC
- Procollagen type I C-peptide
- Tartrate-resistant acid phosphatase (TRACP)
- Brain and CNS
- Cancer and inflammation
- Cell adhesion and ECM
- Environmental hazards
- Epigenetic antibodies
- Metabolic diseases
- mTOR signaling
- Signal transduction
- Stem cell research antibodies
- Virus research
- Miscellaneous research areas
Osteocalcin ELISA kit: Gla-type osteocalcin (Gla-OC) EIA Kit
The Takara Bio osteocalcin ELISA kit offers sensitive detection of the osteogenic marker osteocalcin (OC). OC, also known as gamma-carboxyglutamic acid protein, is a small (5.9 kDa), vitamin K-dependent, hydroxyapatite (Ca2+)-binding protein synthesized exclusively by osteoblasts and odontoblasts. The tissue-specific expression of OC makes it an excellent osteogenic marker for measuring osteoblast activity during bone formation.
The Takara Bio osteocalcin ELISA kit offers sensitive detection of the osteogenic marker osteocalcin (OC). OC, also known as gamma-carboxyglutamic acid protein, is a small (5.9 kDa), vitamin K-dependent, hydroxyapatite (Ca2+)-binding protein synthesized exclusively by osteoblasts and odontoblasts. The tissue-specific expression of OC makes it an excellent osteogenic marker for measuring osteoblast activity during bone formation. Osteocalcin is also a hormone that controls the regulation of glucose and fat deposition and plays a role in male fertility. Three gamma-carboxyglutamic acid (Gla) residues at positions 17, 21, and 24 of the protein are responsible for binding calcium. OC calcium binding is also required for such biological activities as activation of the blood coagulation cascade. Carboxylated OC (Gla-OC) is most likely the active form of the protein, while decarboxylated OC (Glu-OC)—which has weak hydroxyapatite affinity—represents the inactive form. Gla-OC versus Glu-OC serum levels correlate well with bone formation; thus, the Takara Bio human osteocalcin ELISA kit provides a better analysis of bone growth and remodeling than conventional assays that do not differentiate between the active and inactive protein forms.
The Gla-Type Osteocalcin (Gla-OC) EIA Kit is a 96-well in vitro enzyme immunoassay kit for the quantitative determination of human Gla-OC in serum, cultured cell extract, cell culture supernatant, and other biological fluids. It is a solid phase sandwich EIA utilizing two mouse monoclonal Gla-OC antibodies: one of which is coated onto the plate, and the other of which is peroxidase-labeled. This assay format permits highly sensitive detection of Gla-OC using a two-step incubation method. In the first step, biological samples are incubated in the antibody-coated microtiter plate; in the second step, the plate is washed and incubated with the peroxidase-labeled Gla-OC antibody. A substrate is added, and the reaction between the peroxidase and substrate (H2O2, TMBZ) results in color development. The amount of sample Gla-OC is determined by absorbance measurement using an EIA plate reader. Accurate Gla-OC sample concentration can be determined by comparing its specific absorbance with that of the supplied standard on a standard curve.
- Human osteocalcin ELISA
- Highly sensitive quantitative measurement of human Gla-OC
- Quantitative measurement of human, bovine, rabbit, sheep, canine, or goat carboxylated osteocalcin
- Antibody-coated microtiter plage (8 wells × 12 strips)
- Antibody-peroxidase conjugate (lyophilized) (11 ml)
- Standard (8 ng)
- Sample diluent (2 × 11 ml)
- Substrate solution (12 ml)
- Range of assay: 0.5–16 ng/ml
- Assay sensitivity: 0.5 ng/ml
- Assay duration: 3.5 hours
- Specificity: Human Gla-OC. No detectable cross-reactivity with Glu-OC.
- Species cross-reactivity: In addition to human Gla-OC, also cross-reacts with bovine, rabbit, sheep, canine, and goat Gla-OC. No reactivity with mouse and rat Gla-OC. Applications for other species have not been tested.
- Test specimen type: Serum, plasma, culture supernatant, and cell extract.
- Sample volume per well: 100 µl
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Shimizu, N. et al. Shift of serum osteocalcin components between cord blood and blood at day 5 of life. Pediatr. Res. 52, 656–9 (2002).
Tsukamoto, Y., Ichise, H., Kakuda, H. & Yamaguchi, M. Intake of fermented soybean (natto) increases circulating vitamin K2 (menaquinone-7) and gamma-carboxylated osteocalcin concentration in normal individuals. J. Bone Miner. Metab. 18, 216–22 (2000).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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