ELISA wash buffer and stop solution for HRP-based ELISAs
The Wash and Stop Solution for ELISA without Sulfuric Acid contains all of the reagents necessary for preparing wash buffer (10X PBS and Tween 20) and a non-sulfuric acid formulation stop solution for peroxidase-based ELISA or immunoassay applications, including Takara Bio’s EIA kits. The ELISA Wash Buffer components allow for the preparation of both detergent-free and Tween 20-containing wash solutions. The ELISA Stop Solution included with this product is intended for stopping the colorimetric reaction of 3,3′,5,5′- tetramethylbenzidine (TMB) substrate in horseradish peroxidase (HRP)-based ELISA detection. This Stop Solution does not contain the conventional reaction stop reagent, sulfuric acid (H2SO4). It is less hazardous than conventional sulfuric-acid containing stop solutions, yet effectively halts color development for peroxidase reactions with TMB substrate.
- High quality, reliable stock solutions for preparing wash buffers for ELISA or immunoassay protocols.
- Use the Stop Solution directly for halting the color development reaction after addition of the TMB substrate in HRP-based ELISA protocols.
|10X PBS||50 ml x 5|
|Tween 20||3 ml|
|Stop Solution||60 ml|
Note: Precipitate may form if 10X PBS is stored at a low temperature. If this occurs, dissolve precipitates completely by incubating in a warm water bath before use.
- PBS wash buffer: Dilute 50 ml of 10X PBS by adding 450 ml of distilled water to make 1X PBS and mix well.
- PBST (PBS + 0.1% Tween 20) wash buffer: Dilute 50 ml of 10X PBS by adding 450 ml of distilled water to make 1X PBS and mix well. Add 500 µl Tween 20 to 500 ml 1X PBS and mix well.
- Use directly
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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