Challenges in the development and validation of molecular diagnostic assays

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Real-time PCR

Guest webinar

In response to pandemic-related supply chain failures, diagnostic laboratories validated the performance of leading RT-qPCR enzymes, including the One Step PrimeScript III RT-PCR Kit, for the detection of SARS-CoV-2.

In this webinar, Dr. Divya Shah, Senior Healthcare Scientist for Virology at Great Ormond Street Hospital for Children in London, UK presents how her department rapidly overcame obstacles to develop and validate the hospital’s in-house COVID testing, and then developed pediatric respiratory panels.

Webinar outline Meet the presenter Register

Webinar outline

Topics covered:

Validation of primer sets and commercially available master mixes for the hospital’s SARS-CoV-2 detection assays
Development of respiratory panels using the One Step PrimeScript III RT-PCR Kit
Use of metagenomics to identify unexpected infections

Meet the presenter

Divya Shah, PhD

Dr. Divya Shah is a Senior Healthcare Scientist for Virology at the Great Ormond Street Hospital in London. Dr. Shah has over 8 years of experience in assay design and development for molecular diagnostic testing. She currently supports the department to provide molecular testing services, including NGS-based diagnostics.

Register

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