The high local trypsin concentration in the column plus the large internal surface area across a short distance ensures almost instant binding and cleavage of proteins and antibodies at room temperature. Complete antibody digestion takes 3–6 minutes as compared to traditional methods that may require overnight digestion.
- Capturem technology
- Antibody purification
- His-tag purification
- Other tag purification
- Phosphoprotein and glycoprotein purification
- Mass spectrometry digestion reagents
- Matchmaker Gold yeast two-hybrid systems
- Expression systems
Protein digestion with Capturem Trypsin
Capturem membranes are a revolutionary technology that consist of porous, high-capacity membranes that can be functionalized with not only proteases (trypsin or pepsin) for digestion but also ligands such as Protein A or G, nickel, and streptavidin for purification. We've assembled these functionalized membranes into a number of different spin-column, multiwell-plate, and large-volume formats to bring you effortless, high-quality results across your protein research workflow.
Featured product: ♦ Capturem Trypsin
High well-to-well reproducibility
A well-characterized protein commonly used as a standard in peptide studies, apomyoglobin (Apo), was used to test the reproducibility of Capturem Trypsin plates. Apo was digested under native conditions using three different wells of Capturem Trypsin 96-well plates. All three digests yielded nearly identical HPLC profiles (Figure 2), thus demonstrating the reliability of the Capturem Trypsin protocol. Here we show the digestion of proteins within ~2–3 minutes using Capturem Trypsin 96-well plates.
Quantitation using Capturem Trypsin
Capturem 96-well trypsin plates allow specific peptide fragments to be detected and quantified in a high-throughput manner (Figure 3) with excellent well-to-well reproducibility. We performed and analyzed the tryptic spin digest of SILuLite. The quantitative analysis showed that Capturem Trypsin digestion yielded surrogate peptides detectable at ng-input amounts (1, 10, and 50 ng).
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