Labeling antibodies (Abs) is one key way researchers expand their utility for many applications. The addition of fluorescence or biotin enables antibodies to be used for a variety of immunochemistry-based techniques, while antibody-drug conjugates are a rapidly growing area of pharmaceutical research. However, traditional conjugation methods require purified starting material and long incubation times. To improve this process, we've adapted Capturem purification technology to enable rapid on-column conjugations that can be performed directly on unpurified starting material, resulting in labeled antibodies in less than 15 minutes.
In the examples below, we demonstrate the labeling of a mouse IgG targeting Cas9 with fluorescein and biotin. The labeled antibody recognizes its antigen, indicating that the epitope is not negatively affected by the labeling process. To view the conjugation protocols and learn how you can perform rapid antibody labeling, fill out the form on the left. On mobile devices the form is in the menu button.