Antibody therapeutics is ever increasing in complexity and demand. Consequently, those supporting research in this area find a constant need for more efficient tools and methods to help with the work of antibody engineering, production, and purification. Capturem Protein A Minipreps, Maxipreps, and 96-well plates—part of the ever-growing Capturem family, which also includes products for his-tagged protein purification—provide unique solutions for antibody purification without resins or the need for incubation steps. Consisting of spinnable affinity columns or plates containing novel high-capacity Protein A nylon membranes (Figure 1), this system enables researchers to bring speed, ease-of-use, flexibility, and high yield to antibody purification and screening.
Our unique, high-capacity membranes have a high surface area, leading to protein binding capacity better than that of resins at 75 mg or more per cm3 of membrane, and yielding 250 µg of protein per column or well in just five minutes. Where resin-based purification requires a significant amount of work and may take up to a few hours to complete, the entire Capturem purification process can be completed in less than five minutes for minipreps and less than 15 minutes for maxipreps and 96-well plates, without any incubation steps required. Combined with the specific antibody-binding properties of Protein A, Capturem technology provides rapid, high-quality purification and screening of monoclonal and polyclonal antibodies, as well as utility in immunoprecipitation and co-immunoprecipitation experiments.