We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Close

  • ‹ Back to Antibody purification and immunoprecipitation
  • IP and Co-IP of cardiac voltage-gated ion channel proteins
  • Tech note: Rapid Protein G-based antibody purification
  • Tech note: thiophilic antibody purification resins
  • Antibody purification and screening with Capturem Protein A
  • Tech note: IP and Co-IP
  • Rapid antibody labeling with Capturem technology
Capturem Protein A product pages Capturem Protein A
Protein research learning center
Home › Learning centers › Protein research › Antibody purification and immunoprecipitation › Antibody purification and screening with Capturem Protein A

Protein research

  • Capturem technology
    • Capturem protocols
    • Capturem tech notes and applications
    • FAQs about Capturem technology
    • Capturem technology citations
    • Capturem posters
  • Antibody purification and immunoprecipitation
    • IP and Co-IP of cardiac voltage-gated ion channel proteins
    • Tech note: Rapid Protein G-based antibody purification
    • Tech note: thiophilic antibody purification resins
    • Antibody purification and screening with Capturem Protein A
    • Tech note: IP and Co-IP
    • Rapid antibody labeling with Capturem technology
  • His-tag purification
    • Purification methods overview
    • TALON resin selection guide
    • Selection guide: His60 resin
    • xTractor Buffer is optimized for superior protein yield
    • Why tag a protein?
    • Tech note: cobalt resin
    • Simplified purification of active, secreted his-tagged proteins
    • Overview: His60
    • Tech note: Capturem technology
    • Tech note: Capturem large volume
    • Magnetic beads
    • FAQs: TALON
    • Protocols
      • Video: Capturem his maxiprep
      • Video: Capturem his miniprep
      • Visual protocol: Capturem his maxiprep
      • Visual protocol: Capturem his miniprep
      • Capturem nickel column reagent compatibility
      • TALON reagent compatibility
      • His60 reagent compatibility
      • TALON: Native vs denaturing purification
      • Protocol: denaturing purification with TALON resin, imidazole elution
      • Protocol: native purification with TALON resin, imidazole elution
      • Protocol: native purification with TALON resin, pH elution
  • Other tag purification
    • Streptavidin-based enrichment using Capturem technology
    • Selection guide: peptide tags
    • Myc-tagged protein purification overview
    • GST-tagged protein purification overview
  • Phosphoprotein and glycoprotein purification
    • Non-tagged protein purification overview
    • Phosphoprotein purification overview
  • Mass spectrometry digestion reagents
    • Capturem technology for trypsin protein digestion
    • Protein and whole-cell lysate digestion with Capturem Trypsin technology
    • Protein digestion with Capturem Pepsin
  • Matchmaker Gold yeast two-hybrid systems
    • Matchmaker Gold Yeast Two-Hybrid System
    • Make your own library for yeast two-hybrid screening
    • Mate and Plate yeast two-hybrid cDNA libraries
    • Aureobasidin A for improved selectable drug resistance in yeast
  • Expression systems
    • Protein expression overview
    • Insect expression overview
    • Mammalian expression overview
    • pHEK293 Ultra expression overview
    • OKT3 expression in mammalian cells
    • Bacterial expression overview
New products
Need help?
Contact Sales
Capturem Protein A product pages Capturem Protein A
Protein research learning center
Tech Note

Rapid screening of hybridoma clones for the best monoclonal antibody using Capturem Protein A technology

Capturem Protein A Miniprep, Capturem Protein A Maxiprep, and Capturem Protein A 96

  • Technology overview: Capturem Protein A high-capacity membranes
  • Five-minute, no-incubation protocol for high-quality antibody purification
  • Improved animal sera antibody binding compared to traditional Protein A resin
  • Fast screening for the best Cas9 monoclonal antibody from hybridoma clones
Introduction Results Conclusions Methods

Introduction  

Antibody therapeutics is ever increasing in complexity and demand. Consequently, those supporting research in this area find a constant need for more efficient tools and methods to help with the work of antibody engineering, production, and purification. Capturem Protein A Minipreps, Maxipreps, and 96-well plates—part of the ever-growing Capturem family, which also includes products for his-tagged protein purification—provide unique solutions for antibody purification without resins or the need for incubation steps. Consisting of spinnable affinity columns or plates containing novel high-capacity Protein A nylon membranes (Figure 1), this system enables researchers to bring speed, ease-of-use, flexibility, and high yield to antibody purification and screening.

Our unique, high-capacity membranes have a high surface area, leading to protein binding capacity better than that of resins at 75 mg or more per cm3 of membrane, and yielding 250 µg of protein per column or well in just five minutes. Where resin-based purification requires a significant amount of work and may take up to a few hours to complete, the entire Capturem purification process can be completed in less than five minutes for minipreps and less than 15 minutes for maxipreps and 96-well plates, without any incubation steps required. Combined with the specific antibody-binding properties of Protein A, Capturem technology provides rapid, high-quality purification and screening of monoclonal and polyclonal antibodies, as well as utility in immunoprecipitation and co-immunoprecipitation experiments.

Figure 1. Capturem Protein A technology. Panel A. Each Capturem Protein A spin column contains a high-capacity Protein A membrane with increased surface area, providing much higher protein binding capabilities per ml of membrane than per ml of resin. Panel B. Capturem Protein A miniprep spin columns and plates accommodate up to 800 µl of antibody sample and a capacity of up to 250 µg antibody per tube or well, respectively. Capturem Protein A maxiprep spin columns (not shown) accommodate up to ~25 ml of antibody sample and have a capacity of up to 2.5 mg antibody per tube.

Results  

Fast, high-capacity membrane-based antibody purification

Capturem Protein A technology removes the effort from antibody purification and screening with a no-waiting workflow that produces high-quality antibodies quickly and easily. With a protocol as simple as that of DNA minipreps, each column or well is equilibrated and then loaded with diluted antibody sample. Antibodies are bound to the membrane, washed, and eluted. Each step is followed by a one-minute spin for a total purification time of just five minutes (Figure 2). Over 80% of the antibody can be eluted with as little as 100 µl elution buffer.

Figure 2. Capturem Protein A Miniprep workflow for antibody purification. Each mini spin column can be loaded with up to 800 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Antibodies are first bound to the membrane, followed by washing with 300 µl of wash buffer, and elution with 300 µl of elution buffer. Each step is followed by spinning the tube for 1 min at 1,000g. The working bed volume of the membrane is <3 µl. This entire miniprep purification is complete in ~5 min.

Animal sera antibody binding with Capturem Protein A vs. resins

The Capturem Protein A purification system allows for flexibility in sample type, handily accommodating animal sera, cell culture media, and other sources. In order to directly test the performance of Capturem Protein A Minipreps versus traditional Protein A resin, seven different animal sera were used as samples for antibody purification with both methods (Figure 3). Standard manufacturers' conditions were used for both, entailing a five-minute Capturem protocol and a 45-minute resin protocol. Samples purified with Capturem Protein A Minipreps showed excellent purity with Coomassie blue staining, and even showed higher yields than those obtained with the Protein A resin.

Antibodies purified from various animal sera with Protein A resin and Capturem Protein A Minipreps

Amount in elution samples (µg)
SampleProtein A ResinCapturem Protein A Membrane
A. Mouse 90 122
B. Sheep 94 207
C. Goat 55 104
D. Rat 42 191
E. Rabbit 70 94
F. Horse 80 251
G. Human 114 180

Figure 3. Antibodies purified from various animal sera with Protein A resin and Capturem Protein A Minipreps. Capturem Protein A Minipreps and Protein A resin were both used to purify antibodies from 250 µl each of the following animal sera: sheep, goat, rat, mouse, human, rabbit, and horse. Manufacturers' protocols were followed for each purification method. Purities of the final elution samples from both sets were analyzed by gel electrophoresis, followed by staining with Coomassie blue. The amount of antibody eluted was determined by measuring absorbance at 280 nm.

Screening for the best monoclonal Cas9 antibody

In addition to high-quality antibody purification, the Capturem Protein A protocol enables quick and easy antibody screening. With this method, screening hybridomas for the best monoclonal antibody becomes a much simpler process, taking advantage of speed and the ability to directly apply antibody supernatant to the Capturem Protein A columns. In the data below (Figure 4), this technology was used to screen different hybridoma clones for the best monoclonal antibody against Cas9. Crude antibody supernatant was purified with Capturem Protein A, elution was done in a low volume in order to yield a concentrated antibody, and the resulting purified anti-Cas9 antibodies were resolved on an SDS-PAGE gel (Panel A). Cell lysates expressing low and high amounts of Cas9 (Lanes 1 and 2, respectively) were resolved by electrophoresis and transferred to nitrocellulose membranes. These membranes were then blotted using the anti-Cas9 antibodies purified above (Panel B). From these results, two clones were selected for blotting against dilutions of the Cas9 protein (Panel C). With just five minutes of hands-on time, Capturem Protein A maintained excellent results, providing pure, active antibodies for streamlined screening of hybridoma clones.

Figure 4. Screening for the best monoclonal antibody against Cas9 from hybridoma supernatants. All clones were of the mouse IgG1 isotype, and thus purified using a high-salt method. Capturem Protein A maxi columns were equilibrated with buffer and centrifuged. Antibody supernatant was then loaded on a Capturem Protein A maxi column, followed by centrifugation. The loading process was repeated with the flowthrough to maximize antibody binding. The column was then washed with wash buffer, centrifuged, and eluted with 0.5 ml of elution buffer into the collection tube, and neutralized. Panel A. Antibodies purified from cell culture supernatants using Capturem columns were resolved on an SDS-PAGE gel. Panel B. Cell lysates expressing low (Lane 1) or high (Lane 2) amounts of Cas9 were resolved by gel electrophoresis and transferred to nitrocellulose membranes. The membranes were blotted using the antibodies purified with the maxi columns. Panel C. Cas9 protein dilutions (from left to right: 20, 10, 5, 2.5, 1.25, and 0.625 ng), blotted with the selected antibodies.

Conclusions  

Capturem Protein A technology provides researchers with a unique solution to save time and hassle in antibody research. With an incubation- and resin-free workflow, antibody purification is complete in record time while still yielding high-quality, concentrated antibodies. Consisting of single-use spin columns containing high-capacity membranes with mobilized Protein A, this novel system is easy to use and reduces the risk of contamination and carryover. Hybridoma screening also benefits from this fast protocol, with minimal hands-on time and the ability to apply supernatants directly to the columns, thus streamlining the whole process. Capturem Protein A is appropriate for use with antibodies expressed in mammalian cells and whole serum, is compatible with various lysis buffers, and miniprep, maxiprep, and 96-well formats available.

Methods  

Animal sera antibody binding test

Capturem Protein A Miniprep columns were first equilibrated with 800 µl of Protein A binding buffer (0.5 M phosphate, pH 8.0 containing 2 M NaCl), and then centrifuged at 1,000g for 1 min. Serum samples from sheep, goat, rat, mouse, human, rabbit, and horse (250 µl each) were diluted in 1 ml of Protein A binding buffer, and 600 µl of sample was loaded on an equilibrated column, followed by centrifugation at at 1,000g for 1 min. The loading process was then repeated with another 600 µl sample. The columns were then washed with 800 µl of Protein A binding buffer at 1,000g for 1 min. The bound antibody was then eluted with 300 µl of elution buffer (0.1 M glycine, pH 2.5) into a tube containing 30 µl of neutralization buffer (1 M tris, pH 8.5) to neutralize the eluted antibody.

For the resin (Thermo Fisher Scientific), 20 µl of slurry was used for each purification, following the vendor's protocol for antibody purification. Storage buffer was first removed, followed by equilibration of the beads with Protein A binding buffer (Thermo Fisher Scientific; 600 µl x 2). The equilibration buffer was then removed and the beads were incubated with diluted serum samples (1 ml of serum in 3 ml of buffer, 1.2 ml of diluted sample) for 4 min with end-over-end rotation. Following loading, the resin was washed twice with 600 µl of Protein A binding buffer, drained, and eluted with 600 µl of elution buffer plus 60 µl of neutralization buffer in a collection tube.

The eluates from various sera were resolved by gel electrophoresis and stained with Coomassie blue. Absorbance at 280 nm was measured with a NanoDrop 2000 spectrophotometer, and used to quantify the amount of antibody in eluted fractions.

Screening for the best monoclonal antibody against Cas9 from hybridoma supernatants

All clones were of the mouse IgG1 isotype, and thus purified using the high-salt method as detailed below. Capturem Protein A maxi columns were equilibrated with 6 ml of buffer (10 mM sodium borate, pH 8.9, 3 M NaCl), and centrifuged at 2,000g for 3 min. The NaCl concentration of the crude antibody supernatant was adjusted to 3.3 M, and to this, 1/10 of its volume of 1.0 M sodium borate (pH 8.9) was added. The supernatant (20 ml) was then loaded on a Capturem Protein A maxi column, followed by centrifugation at 2,000g for 3 min. The loading process was repeated with the flowthrough to maximize antibody binding. The column was then washed with 10 ml of wash buffer (3 M NaCl, 10 mM sodium borate, pH 8.9), centrifuged, and eluted with 0.5 ml of elution buffer (100 mM glycine, pH 3.0). The eluate was neutralized by adding 50 µl of 1 M Tris, pH 8.5, into the collection tube. Antibodies purified from cell culture supernatants using Capturem columns were resolved on an SDS-PAGE gel. Cell lysates expressing low or high amounts of Cas9 were resolved by gel electrophoresis and transferred to nitrocellulose membranes. The membranes were blotted using the antibodies purified with the maxi columns. Cas9 protein dilutions (20, 10, 5, 2.5, 1.25, and 0.625 ng), were blotted with the selected antibodies.

Related Products

Cat. # Product Size License Quantity Details
635720 Capturem™ Protein A Maxiprep Columns 6 Columns USD $320.00

License Statement

ID Number  
273 This product is covered by U.S. Patent Nos. 9,459,188 and/or 10,207,229, exclusively licensed to Takara Bio USA, Inc.

Capturem Protein A Maxiprep is a single-use disposable column for simple, rapid purification of antibodies that yields up to 1 mg of purified antibody per column, depending upon antibody isotype and species. This maxiprep column is suitable for purification of antibodies from animal sera, ascites fluid, cell culture media and other sources. Each column can hold up to 23 ml of sample and requires a minimum elution volume of 1.5 ml.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

Capturem Protein A- or G-functionalized membranes

Capturem Protein A- or G-functionalized membranes

Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.

Back

Capturem spin columns and filtration devices

Capturem spin columns and filtration devices

Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.

Back

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and yields of Capturem purification formats.

Back

635720: Capturem Protein A Maxiprep Columns

635720: Capturem Protein A Maxiprep Columns
635717 Capturem™ Protein A Miniprep Columns 12 Columns USD $215.00

License Statement

ID Number  
273 This product is covered by U.S. Patent Nos. 9,459,188 and/or 10,207,229, exclusively licensed to Takara Bio USA, Inc.

Capturem Protein A Miniprep is a single-use disposable column for simple, rapid purification of antibodies that yields up to 40 μg of purified antibody per column, depending upon species and antibody isotype. This miniprep column is suitable for purification of antibodies from animal sera, ascites fluid, cell culture media and other sources. Each column can hold up to 850 µl of sample and requires a minimum elution volume of 100 µl.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

Capturem Protein A mini spin columns were tested for the ability to purify antibodies from various animal sera: sheep, goat, rat, mouse, human, rabbit, and horse

Capturem Protein A mini spin columns were tested for the ability to purify antibodies from various animal sera: sheep, goat, rat, mouse, human, rabbit, and horse
Capturem Protein A mini spin columns were tested for the ability to purify antibodies from various animal sera: sheep, goat, rat, mouse, human, rabbit, and horse. Columns were first equilibrated with 800 μl of Protein A binding buffer (0.5 M phosphate, pH 8.0 containing 2 M NaCl), and then centrifuged at 1,000 x g for 1 min. Serum samples (250 μl) were diluted in 1 ml of Protein A binding buffer, and 600 μl of sample was loaded on an equilibrated column, followed by centrifugation at at 1,000 x g for 1 min. The loading process was then repeated with another 600-μl sample. The columns were then washed with 800 μl of Protein A binding buffer at 1,000 x g for 1 min. The bound antibody was then eluted with 300 μl of elution buffer (0.1 M glycine, pH 2.5) into a tube containing 30 μl of neutralization buffer (1 M Tris, pH 8.5) to neutralize the eluted antibody. The eluates from various sera were resolved by gel electrophoresis and stained with Coomassie blue. Absorbance at 280 nm was measured with a NanoDrop 2000 spectrophotometer, and used to quantify the amount of antibody in eluted fractions.

Back

Capturem Protein A Miniprep workflow for antibody purification

Capturem Protein A Miniprep workflow for antibody purification
Capturem Protein A Miniprep workflow for antibody purification. Each mini spin column can be loaded with up to 800 μl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Antibodies are first bound to the membrane, followed by washing with 300 μl of wash buffer, and elution with 300 μl of elution buffer. Over 80% of the antibody can be eluted with as little as 100 μl of elution buffer. Each step is followed by spinning the tube for 1 min at 1,000 x g. The working bed volume of the membrane is <2 μl. This entire purification is complete in ~5 min

Back

Capturem Protein A miniprep and 96-well plate

Capturem Protein A miniprep and 96-well plate
Capturem Protein A miniprep and 96-well plate. Capturem Protein A miniprep spin columns accommodate up to 800 μl of antibody sample, and have a capacity of up to 300 μg, depending upon the antibody isotype. Capturem 96-well plates accommodate up to 800 μl of antibody sample per well, with a capacity of up to 300 μg immunoglobulin per well.

Back

Capturem Protein A mini spin columns were compared against a Protein A resin

Capturem Protein A mini spin columns were compared against a Protein A resin
Capturem Protein A mini spin columns were compared against a Protein A resin. Sera from the following species were tested: mouse, sheep, goat, rat, rabbit, horse, and human. For the resin (Thermo Fisher Scientific), 20 μl of slurry was used for each purification, following the vendor’s protocol for antibody purification. Storage buffer was first removed, followed by equilibration of the beads with Protein A binding buffer (Thermo Fisher Scientific; 600 μl x 2). The equilibration buffer was then removed and the beads were incubated with diluted serum samples (1 ml of serum in 3 ml of buffer, 1.2 ml of diluted sample) for 4 min with end-over-end rotation. Following loading, the resin was washed twice with 600 μl of Protein A binding buffer, drained, and eluted with 600 μl of elution buffer plus 60 μl of neutralization buffer in a collection tube. Purification steps for the Capturem samples were performed as described in the Capturem Protein A Miniprep Protocol-At-A-Glance. Serum samples were the same concentration and dilution as those used for the Protein A resins. Purities of the final elution samples from both sets were analyzed by gel electrophoresis and stained with Coomassie blue. The amount of antibody eluted was determined by measuring absorbance at 280 nm.

Back

Capturem Protein A- or G-functionalized membranes

Capturem Protein A- or G-functionalized membranes

Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.

Back

Capturem spin columns and filtration devices

Capturem spin columns and filtration devices

Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.

Back

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and yields of Capturem purification formats.

Back

635717: Capturem Protein A Miniprep Columns

635717: Capturem Protein A Miniprep Columns


Capturem membrane technology

Capturem technology

There is a constant need for faster, more efficient antibody and protein purification processes at any scale. Capturem membrane technology allows for purification directly from complex matrices, such as cell supernatants, in minutes. This technology also provides highly purified and concentrated antibodies and his-tagged proteins, even from samples containing additives not compatible with other purification technologies.

Antibody purification His-tagged purification

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2022 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • PCR and qPCR hot summer sale
  • Cell-free DNA purification kit sale
  • GoStix Plus special offers
  • PCR samples
  • ThruPLEX HV special offer
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • OEM enzyme FAQs
  • Custom enzyme samples
  • Exploring OEM and custom enzyme partnerships
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • cDNA synthesis
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody purification and immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • DNA Day 2022
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Services & Support
  • Learning centers
  • Applications
  • About
  • Contact us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • Diagnostic solutions
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Real-time PCR
  • mRNA and cDNA synthesis
  • COVID-19 research
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Epigenetics and small RNA sequencing
  • NGS accessories
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • ProteoTuner protein control systems
  • iDimerize inducible protein interaction systems
  • Transfection reagents
  • Mammalian expression plasmids
  • Cell biology assays
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • Accessories
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Expression vectors & systems
  • Glycobiology
  • Antibodies and immunoprecipitation
  • SDS-PAGE & western blotting
  • Protein sequencing
  • Accessory enzymes
  • Diagnostic solutions
  • Infectious diseases
  • Reproductive health
  • PCR
  • Most popular polymerases
  • Standard PCR
  • High-yield PCR
  • High-fidelity PCR
  • Fast PCR
  • Long-range PCR
  • GC rich PCR
  • Direct PCR
  • PCR master mixes
  • Custom business friendly and automation-ready solutions
  • Molecular diagnostic products
  • GMP-grade products
  • Application-specific PCR
  • Other PCR-related products
  • PCR thermal cyclers
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Mutagenesis kits
  • Ligation enzymes
  • Restriction enzymes
  • Modifying enzymes
  • X-Gal and IPTG
  • Linkers, primers, and cloning vectors
  • Agarose gel electrophoresis
  • Nucleic acid extraction
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • RNA cleanup kits
  • Cell-free DNA purification kits
  • Viral DNA and RNA purification kits
  • Microbiome
  • Accessories and components
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Secondary antibodies
  • Antibody and ELISA accessories
  • Fluorescent protein antibodies
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • Real-time PCR primer sets
  • References and standards for qPCR
  • RNA extraction and analysis for real-time qPCR
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • cDNA synthesis accessories
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Services & Support
  • Instrument services
  • OEM & custom enzyme manufacturing
  • Stem cell services
  • Gene and cell therapy manufacturing services
  • Customer service
  • Technical support
  • Sales
  • Shipping & delivery
  • Partnering & Licensing
  • Feedback
  • Webinars from Takara Bio
  • Vector information
  • Online tools
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • OEM enzyme FAQs
  • Custom enzyme samples
  • Exploring OEM and custom enzyme partnerships
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Webinars from Takara Bio
  • NGS: biomarkers and oncology
  • NGS: immunology
  • Stem cells
  • Real-time PCR
  • Gene function
  • Protein science
  • Vector information
  • Vector document overview
  • Vector document finder
  • Online tools
  • GoStix Plus FAQs
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Real-time PCR
  • cDNA synthesis
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Apollo library prep system introduction
  • Next-generation sequencing
  • Product line overview
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Newsletters
  • Webinars
  • Citations
  • Posters
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Cell biology assays
  • Stem cell research
  • Overview
  • Protocols
  • Applications
  • Technical notes
  • Posters
  • Webinars
  • Videos
  • FAQs
  • Citations
  • Selection guides
  • Protein research
  • Capturem technology
  • Antibody purification and immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Expression systems
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • FAQ
  • Go green with lyophilized enzymes
  • LA PCR technology
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Sign up to stay updated
  • Traditional molecular cloning
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • RNA purification
  • RNA purification kit finder
  • Viral DNA and RNA purification
  • Parallel DNA, RNA & protein
  • Automated DNA and RNA purification
  • Accessory selection guides
  • Microbiome
  • Antibodies and ELISA
  • Osteocalcin focus
  • Real-time PCR
  • Overview
  • Product finder
  • Reaction size guidelines
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • FAQs
  • cDNA synthesis
  • Premium total and poly A+ RNA
  • SMARTer RACE 5'/3' Kit
  • Cloning antibody variable regions
  • Applications
  • Molecular diagnostics
  • Pathogen detection
  • Vaccine development
  • Cancer research
  • Immunotherapy research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Molecular diagnostics
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • DSS Takara Bio India Pvt. Ltd : Manufacturing
  • Our partners
  • Special offers
  • New products
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • DNA Day 2022
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Special offers
  • PCR and qPCR hot summer sale
  • Cell-free DNA purification kit sale
  • GoStix Plus special offers
  • PCR samples
  • ThruPLEX HV special offer
  • Lab Essentials
  • IVTpro mRNA Synthesis kit
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us