Fast PCR selection guide
Don't wait around for results—use fast PCR enzymes and speed up your reactions
Takara Bio's fast DNA polymerases provide higher processing speeds than conventional Taq. Use the tables below to find the perfect fast PCR enzyme to speed up your PCR so you can obtain results sooner. Click on each enzyme to learn more.
| Which fast PCR enzyme is right for your application? | |||
|---|---|---|---|
| PrimeSTAR Max DNA Polymerase |
SpeedSTAR HS DNA Polymerase |
SapphireAmp Fast PCR Master Mix |
|
| cDNA amplification & library construction | ✔ | ||
| Cloning applications | ✔ | ||
| Colony screening | ✔ | ||
| Cycle sequencing | ✔ | ||
| Endpoint RT-PCR | ✔ | ✔ | ✔ |
| Genotyping (SNP) | ✔ | ✔ | |
| High-throughput applications | ✔ | ✔ | ✔ |
| Amplification prior to NGS | ✔ | ||
| Long PCR | ✔ | ✔ | |
| Mutagenesis | ✔ | ||
| Key features of fast PCR enzymes | |||
|---|---|---|---|
| PrimeSTAR Max DNA Polymerase |
SpeedSTAR HS DNA Polymerase |
SapphireAmp Fast PCR Master Mix |
|
| Fidelity | 29X Taq | 4.5X Taq | 3X Taq |
| Speed | 5 sec/kb | 10 sec/kb | 10 sec/kb |
| TA cloning compatible | ✔ | ✔ | |
| Hot start | ✔ | ✔ | ✔ |
| Dye added | ✔ | ||
| Free sample available | ✔ | ✔ | ✔ |
| Notes | Fast, convenient, and highest fidelity | Fast moderate-fidelity PCR | Fast routine PCR & colony PCR |
Tech note
Go from DNA to data with a fast polymerase that's also astonishingly accurate
Perform fast, high-fidelity PCR with shorter extension times using PrimeSTAR Max DNA Polymerase.
See what our customers are saying about our fast DNA polymerases!
I used PrimeSTAR MAX polymerase for mutagenesis PCR. For all the sample, mutations were correctly introduced. It took only two days to obtain mutant clones."
—Fumiaki Ito, UNIV OF SOUTHERN CALIFORNIA
It worked with a fast protocol I have used for a new design of a thermal cycler. My goal is to make PCR as fast as possible to cut down the overall time of a reactions. I wonder if the SpeedSTAR will work well with real-time reaction in the presence of dyes."
—Season Wong, AI BIOSCIENCES INC
I was very satisfied with the SapphireAmp! Colony pcr's that normally take over 3 hours were done in 1 hour! I've already purchased more of this product."
—Nicole Davis, UNIV OF CALIF SAN FRANCISCO
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
