Direct PCR overview

When do you need it?

Direct PCR allows for amplification directly from tissues or crude extracts, without the need for DNA purification. Omitting the multi-step process for preparing genomic DNA helps prevent sample loss, saves time, and reduces cost. Direct PCR vastly simplifies genotyping experiments, especially when the source material is limited.

How is it achieved?

Direct PCR relies on modified polymerases and buffer systems that enable DNA amplification in the presence of PCR inhibitors, growth serum, and other source material components. The table below provides an overview of all the Terra Direct PCR DNA polymerases that Takara Bio offers (Terra is offered as MightyAmp outside US & Canada):

Terra PCR Direct
Format:	Polymerase mix	Red dye premix	Genotyping kit	FFPE kit	FTA card kit
Recommended use:	General purpose; suitable for most applications	Premix with convenient loading	All-in-one kit; most robust performance	Application-specific for FFPE samples	Application-specific for Whatman FTA cards
Contents:	Standard Terra PCR Direct Polymerase Mix	2X master mix containing a specially formulated loading dye	Extraction buffer* and loading dye included	Extraction buffer* included	Does not include Proteinase K
Recommended Sample Types
FFPE
Blood (FTA)
Blood (Heparin)
Plant leaves (FTA)
Plant leaves			*
Buccal cells (FTA)
Buccal cells
Saliva
Tissues			*
Cell pellet
Crude extract
Purified DNA

*The Terra PCR Direct Genotyping Kit includes an extraction buffer, which we recommended using with tough plants or tissue samples.