Direct PCR overview
When do you need it?
Direct PCR allows for amplification directly from tissues or crude extracts, without the need for DNA purification. Omitting the multi-step process for preparing genomic DNA helps prevent sample loss, saves time, and reduces cost. Direct PCR vastly simplifies genotyping experiments, especially when the source material is limited.
How is it achieved?
Direct PCR relies on modified polymerases and buffer systems that enable DNA amplification in the presence of PCR inhibitors, growth serum, and other source material components. The table below provides an overview of all the Terra Direct PCR DNA polymerases that Takara Bio offers (Terra is offered as MightyAmp outside US & Canada):
|Terra PCR Direct|
|Format:||Polymerase mix||Red dye premix||Genotyping kit||FFPE kit||FTA card kit|
|Recommended use:||General purpose; suitable for most applications||Premix with convenient loading||All-in-one kit; most robust performance||Application-specific for FFPE samples||Application-specific for Whatman FTA cards|
||Standard Terra PCR Direct Polymerase Mix||2X master mix containing a specially formulated loading dye||Extraction buffer* and loading dye included||Extraction buffer* included||Does not include Proteinase K|
|Recommended Sample Types|
|Plant leaves (FTA)|
|Buccal cells (FTA)|
*The Terra PCR Direct Genotyping Kit includes an extraction buffer, which we recommended using with tough plants or tissue samples.
Amplifying a gene of interest from raw and processed meat samples
Terra PCR Direct polymerase demonstrates an exceptional ability to provide consistently strong amplification of a target gene directly from raw or processed meat.
Amplifying a gene of interest from human nail DNA
Terra PCR Direct Polymerase Mix allows for direct amplification from a tissue source or crude extracts, without the need for DNA purification.
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