Higher yields than a leading competitor from low-abundance cfDNA samples. Total cfDNA was purified from 2 ml of human EDTA plasma derived from four challenging donor samples known to have a low abundance of cfDNA (<10 ng cfDNA/ml plasma). Purification using the NucleoMag cfDNA kit resulted in higher and more consistent yields of total cfDNA compared to the competitor. The final total DNA recovery was quantified using the Qubit dsDNA High Sensitivity kit on a Qubit fluorometer (Thermo Fisher Scientific).

Consistent recovery of cell-free DNA regardless of plasma volume. cfDNA from human EDTA plasma isolated using the NucleoMag cfDNA kit shows clear bands when analyzed via capillary gel electrophoresis (using the Agilent Bioanalyzer 2100 system with the High Sensitivity DNA kit). cfDNA is clearly detectable, even when it is purified from small volumes of plasma.

DNA yield strongly depends on individual samples, but is typically in the range of 0.1 to 100 ng DNA per ml of plasma. The NucleoMag cfDNA kit was used to isolate cfDNA from human EDTA plasma samples ranging from 2–10 ml in volume. Efficient purification of cfDNA was indicated by the characteristic peak at approximately 170 bp (using the Agilent Bioanalyzer 2100 system and the High Sensitivity DNA kit). The total yield was determined by PicoGreen quantification assay using the Quant-iT PicoGreen kit (Thermo Fisher Scientific) on a Synergy HT Multi-detection microplate reader (Biotek). The consistent correlation of cfDNA amount and sample volume demonstrated reliable performance independent of sample volume.