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  • ‹ Back to Single-cell RNA- and DNA-seq
  • All-in-one cDNA synthesis and library prep from single cells
  • Highest sensitivity for single-cell mRNA-seq
  • Stranded libraries from single cells
  • Streamlined single-cell mRNA-seq
  • Full-length mRNA libraries from single cells (SMART-Seq v4)
  • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
  • Full-length mRNA libraries from single cells for Fluidigm C1 (SMART-Seq v4)
  • Full-length single-cell library method comparison
  • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
  • Next-gen WGA method for CNV and SNV detection from single cells
SMARTer NGS SMART-Seq Single Cell Kit product information
Isoform analysis Customer data: combining droplet and full-length sequencing
SSsc outperforms SS2 Customer data: smashing single-cell sensitivity
Single cell 5 FACS tips for scRNA-seq
Single cells 5 tips for successful scRNA-seq experiments
Home › Learning centers › Next-generation sequencing › Technical notes › Single-cell RNA- and DNA-seq › Highest sensitivity for single-cell mRNA-seq

Technical notes

  • Single-cell RNA- and DNA-seq
    • All-in-one cDNA synthesis and library prep from single cells
    • Highest sensitivity for single-cell mRNA-seq
    • Stranded libraries from single cells
    • Streamlined single-cell mRNA-seq
    • Full-length mRNA libraries from single cells (SMART-Seq v4)
    • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
    • Full-length mRNA libraries from single cells for Fluidigm C1 (SMART-Seq v4)
    • Full-length single-cell library method comparison
    • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
    • Next-gen WGA method for CNV and SNV detection from single cells
  • RNA-seq
    • Stranded libraries from picogram-input total RNA (v3)
    • Automation-friendly, all-in-one cDNA synthesis and library prep
    • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
    • Stranded libraries from picogram-input total RNA (v2)
    • Stranded libraries from FFPE inputs (v2)
    • Stranded libraries from 100 ng - 1 ug total RNA
    • Stranded libraries from 100 pg-100 ng total RNA
    • Stranded libraries from picogram-input total RNA (v1)
    • Stranded RNA-seq competitor kit comparison
    • Nonstranded libraries from FFPE inputs
    • Sensitive capture of full-length transcript information with targeted RNA-seq
  • DNA-seq
    • ThruPLEX HV outperforms NEBNext Ultra II
    • Accurate detection of low-frequency variants using molecular tags
    • Streamlined DNA-seq from challenging samples
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    • Sequencing analysis of low-frequency mutations in cfDNA
    • DNA-seq from FFPE samples
    • Low-input whole-exome sequencing
    • Tag-seq variant detection
    • Low-volume DNA shearing for SMARTer ThruPLEX library prep
  • Immune profiling
    • BCR repertoire profiling from human samples (bulk)
    • Improved TCR repertoire profiling from human samples (bulk)
    • TCR repertoire profiling from human samples (single cells)
    • TCR repertoire profiling from human samples (bulk)
    • TCR repertoire profiling from mouse samples (bulk)
    • BCR repertoire profiling from mouse samples (bulk)
  • Epigenetics and smRNA-seq
    • Full-length small RNA libraries
    • ChIP-seq libraries for transcription factor analysis
    • ChIP-seq libraries from ssDNA
    • Methylated DNA-seq
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SMARTer NGS SMART-Seq Single Cell Kit product information
Isoform analysis Customer data: combining droplet and full-length sequencing
SSsc outperforms SS2 Customer data: smashing single-cell sensitivity
Single cell 5 FACS tips for scRNA-seq
Single cells 5 tips for successful scRNA-seq experiments
Tech Note

Unprecedented sensitivity with the SMART-Seq Single Cell Kit

  • Optimized chemistry for single-cell inputs, particularly those with very low RNA content
  • Unparalleled sensitivity leading to lower dropout rates and higher reproducibility
  • Higher cDNA yield associated with more genes identified
  • Highly scalable workflow that is easily automated
  • Outperforms the NEBNext single-cell protocol
Introduction Results Conclusions Methods References

Introduction  

The human body is made of trillions of cells, partitioned into hundreds of different types and subtypes that we can now characterize in high definition. Advances in library preparation and sequencing technologies have allowed the single-cell analysis community to investigate the content of each cell more accurately than ever. Currently, droplet sequencing is the primary method used to survey these cells, as it allows the capture of transcriptomes from thousands of cells in parallel and is useful for cell-type identification. However, since the droplet-based method focuses on single mRNA ends (typically the 3' end), it gathers limited information. Complementary methods that provide full-length mRNA information, such as the Smart-seq2 method (Picelli et al. 2013) or Takara Bio's SMART-Seq® v4 Ultra Low Input RNA Kit for Sequencing (SSv4), are preferred for generating richer datasets. Using full-length and droplet-based methods in parallel is an emerging need in the scientific community, especially for consortia such as the Human Cell Atlas. There is still room for improvement of the Smart-seq2 method, particularly for use with challenging samples (e.g., cells with very low RNA content or nuclei). To address this need, we have further modified our core SMART-Seq technology to create a new chemistry with higher sensitivity designed specifically for single-cell applications—the SMART-Seq Single Cell Kit (Figure 1)—which outperforms all currently available full-length methods.

SMART-Seq Single Cell Kit workflow

Figure 1. SMART-Seq Single Cell Kit technology and workflow. The SMART-Seq Single Cell Kit's plate-based workflow allows direct input of single cells isolated by FACS or other methods. SMART technology (Chenchik et al. 1998) is then used in a ligation-free protocol to generate full-length cDNA. The reverse transcriptase (RT) adds nontemplated nucleotides (indicated by Xs) that hybridize to the SMART-Seq sc TSO, providing a new template for the RT. Chemical modifications to block ligation during sequencing library preparation (if using a ligation-based library preparation method) are present on some primers (indicated by the black stars). The SMART adapters, added by the oligo(dT) primer (3′ SMART-Seq CDS Primer II A) and SMART-Seq sc TSO, are indicated in green and used for amplification during PCR. The amplified cDNA is then purified, quantified, and used for sequencing library preparation with the SMART-Seq Library Prep Kit (sold as part of the SMART-Seq Single Cell PLUS Kit).

Results  

Greater sensitivity and reproducibility than Smart-seq2

The Smart-seq2 protocol (Picelli et al. 2013) and Takara Bio's SMART-Seq technology are widely used by the scientific community to generate in-depth characterization of the transcriptome at the single-cell level. To compare performance between the new SMART-Seq Single Cell Kit (SSsc) and the Smart-seq2 protocol, we processed single cells from the lymphoblastoid cell line GM12878 according to each chemistry's protocol (Figure 2).

We observed that the read distribution is different between the two chemistries. The Smart-seq2 chemistry had a much higher number of reads mapping to the mitochondrial genome (Figure 2, Panel A), resulting in a lower number of reads available for gene identification. We also observed a 15% increase in the number of genes identified in the cells processed with SSsc as compared to Smart-seq2 (Figure 2, Panel B). The higher sensitivity of the SSsc method is associated with greater reproducibility across cells, as indicated by the higher Spearman correlation within SSsc-processed cells (Figure 2, Panel C) and a lower dropout rate than Smart-seq2 (Figure 2, Panel D).

SMART-Seq Single Cell Kit outperforms Smart-seq2

Figure 2. The SMART-Seq Single Cell Kit outperforms the Smart-seq2 protocol. Single cells from the lymphoblastoid cell line GM12878 were processed with SSsc (18 cells) or the Smart-seq2 protocol (20 cells; Picelli et al. 2014) using 19 cycles of PCR. As described in the methods, RNA-seq libraries were generated and sequences analyzed (after normalizing all samples to 1.75 million paired-end reads). Panel A. The read distribution varied between the two chemistries, with increased mitochondrial reads using Smart-seq2 and increased exonic reads using SSsc. Panel B. Boxplots, where the boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles, for each method; the whiskers are 1.5 x IQR from the median value and represent the extremes of the data. More genes are detected for cells processed with SSsc (median = 9,980) versus Smart-seq2 (median = 8,732). Panel C. Boxplots of the Spearman's correlation results between all cells for each method plotted, with the same IQR as in Panel B. These plots show there is a higher correlation among the cells processed with SSsc (median = 0.82), which indicates a greater reproducibility than the Smart-seq2 method (median = 0.69). Panel D. The greater reproducibility of SSsc is also demonstrated by the lower dropout rate of the genes detected with a TPM >1.

Greater sensitivity and reproducibility than SSv4 for single cells

The SSv4 kit is the most sensitive single-cell RNA-seq method available, and considered by many as the gold standard for plate-based full-length scRNA-seq (Hodge et al. 2019; Ibrayeva et al. 2019; and other references). Through several experiments, we were able to demonstrate that the new SSsc kit generates data with even superior sensitivity and reproducibility than the SSv4 kit.

For the first experiment, we used 2 pg of high-quality control RNA to compare the two kits (Table 1). We observed a dramatically higher cDNA yield with the SSsc kit. When looking at three technical replicates, the SSsc kit generated on average a total of 13.1 ng of cDNA, while the SSv4 kit generated 6.7 ng, representing a twofold increase. Following sequencing and data analysis, we found that the SSsc kit identified about 15% more genes than SSv4. In addition, SSsc produced superior reproducibility, as evidenced by the increased Pearson and Spearman correlations (Figure 3). These scatter plots show that SSsc was able to detect additional genes that were expressed at a low level.

Sequencing metrics comparing the SMART-Seq v4 kit and SMART-Seq Single Cell Kit
RNA source 2 pg UHR total RNA
cDNA synthesis method SSv4 SSsc
Replicate A B C A B C
cDNA yield (ng) 7.8 6.9 5.5 14.8 14.9 9.6
Number of genes with TPM >1 7,412 7,522 7,487 8,774 8,614 8,406
Number of genes with TPM >0.1 8,660 8,868 9,240 10,319 10,276 10,285
Average Pearson/Spearman 0.95/0.59 0.97/0.63
Proportion of reads mapped (%):
Genome 92.7 92.5 92.5 80.1 80.9 80.6
Exon 79.3 78.7 76.6 63.4 64.1 62.0
Intron 10.5 10.9 12.5 13.0 12.8 14.0
Intergenic regions 2.9 3.0 3.4 3.7 4.0 4.6
rRNA 0.8 0.7 0.6 6.1 6.0 4.3
Mitochondria 3.5 3.6 3.9 9.3 8.4 10.2

Table 1. Increased sensitivity with the SMART-Seq Single Cell Kit. Replicate cDNA libraries were generated from 2 pg of Universal Human Reference (UHR) total RNA using the SMART-Seq v4 kit (SSv4) or the SMART-Seq Single Cell Kit (SSsc); all libraries were processed with 19 PCR cycles. As described in the methods, RNA-seq libraries were generated and sequences analyzed (after normalizing all samples to 1.6 million paired-end reads). SSsc identified about 15% more genes than SSv4.

SMART-Seq Single Cell Kit increased reproducibility over SMART-Seq v4 kit

Figure 3. Increased reproducibility with the SMART-Seq Single Cell Kit. Libraries made from 2 pg of UHR total RNA (Table I) were analyzed using scatter plots to visualize the reproducibility between technical replicates (shown are TPM values from all genes with a log10+1 scale). SSv4 (Panel A) generated highly reproducible quantification, but SSsc (Panel B) produced superior reproducibility, as seen in the increased Pearson and Spearman correlations. In addition, SSsc was better at detecting low-expression genes.

In a second experiment, the performance of the SSsc kit was further evaluated using FACS-sorted cells. Single cells from lymphoblastoid cell line GM22601 (Figure 4) or the PBMC population from a healthy donor (Figure 5) were processed with SSv4 and SSsc. As seen in the first experiment using the UHR total RNA, the cDNA yield was dramatically higher with the SSsc kit (Figure 4, Panel A; data not shown for PBMCs). We continued to observe the SSsc kit's improved performance in the sequencing data generated from both cell types. First, we observed that the read distribution was comparable between the two kits (Figure 4, Panel B and Figure 5, Panel A). Second, we found that we could identify more genes with the SSsc kit: ~50% increase in the GM22601 cell line (Figure 4, Panel C) and ~60% increase in the PBMC population (Figure 5, Panel B). This dramatic increase in sensitivity with the SSsc kit held true over a wide range of sequencing depths (Figure 5, Panel B). For the relatively homogeneous cell population of the GM22601 line, the SSsc kit was more reproducible in terms of expression levels across all genes in the 12 cells analyzed, as shown by the higher Spearman correlation (Figure 4, Panel D)—in accordance with the data obtained using UHR total RNA (Table 1).

SSsc outperforms SSv4 with lymphoblastoid cells

Figure 4. Improved performance for single cells with low RNA content. 12 single cells from lymphoblastoid cell line GM22601 were processed with SSv4 or SSsc using 19 cycles of PCR. As described in the methods, RNA-seq libraries were generated and sequences analyzed (after normalizing all samples to 1.25 million paired-end reads). For all boxplots in this figure, the box denotes the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5 x IQR from the median value and represent the extremes of the data. Panel A. The cDNA yield generated with SSsc (median = 28.8 ng) is significantly higher than that generated with SSv4 (median = 6.6 ng). Panel B. The read distribution was fairly similar between the two chemistries. Panel C. Over 50% more genes were detected in the cells processed with SSsc (median = 9,980) relative to SSv4 (median = 6,810). Panel D. Correlation boxplots show higher intragroup Spearman correlation between all cells processed with SSsc, indicating greater reproducibility for the SSsc method (median = 0.68) relative to SSv4 (median = 0.51).

SSsc outperforms SSv4 with PBMCs

Figure 5. Improved performance with primary samples. PBMCs from a healthy donor were processed with the SSv4 or SSsc kit (~50 single cells per kit). RNA-seq libraries were generated as described in the methods. Panel A. The read distribution is fairly similar between the two chemistries. Panel B. About 60% more genes are detected in the cells processed with SSsc, regardless of the number of reads used for the analysis.

SSsc outperforms the NEBNext single-cell protocol

Like SSsc, the NEBNext Single Cell/Low Input Kit (NEBNext) is a method to generate RNA-seq libraries from single cells. Both technologies are based on RT with a poly-A targeting primer, template switching for cDNA library generation, and PCR amplification for high library yield. Both SSsc and NEBNext are intended to produce unbiased and uniform libraries from simple, user-friendly workflows.

NEBNext is promoted as a method that provides high-quality sequencing data from single cells resulting in unmatched detection of low-abundance transcripts. Moreover, it is advertised as a robust and highly sensitive method.

In order to test these performance parameters between SSsc and NEBNext, we performed single-cell analysis using each kit according to the manufacturer's instructions.

Three charts data charts comparing the NEBNext protocol results to that of SSsc

Figure 6. The SMART-Seq Single Cell Kit outperforms the NEBNext protocol. Libraries were prepared from T cells according to manufacturer's instructions with either SSsc or NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina. Panel A. In these boxplots, the box denotes the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5 x IQR from the median value and represent the extremes of the data. The number of genes identified with a TPM >0.1 is higher (~40%) for SSsc (median = 3,591) than for NEBNext (median = 2,665). Panel B. The read distribution is different between the two chemistries, with more reads mapping to exon regions for SSsc. Panel C. The high number of cycles required to generate a library for the NEBNext protocol means that the negative controls for NEB (red) cluster strongly with the single-cell libraries (light blue), while the negative controls for SSsc (purple) are distinct from the single-cell libraries (dark blue).

While NEBNext cDNA yield was higher, this did not translate to a higher number of genes identified. Even with a lower cDNA yield, SSsc identified 40% more genes, allowing for a greater depth of understanding of the sample. Read distributions demonstrate SSsc has a higher percentage of exon-mapped reads and a lower percentage of intronic reads. As consistently seen in SSsc data sets, SSsc does have a slightly higher percentage of rRNA mapping, but this is due to the increased sensitivity of SSsc to allow for the optimized capture of information from single cells. It should be noted that despite the slightly higher rRNA, the number of genes identified is still much greater for SSsc. In Figure 6, Panel C, we continue to compare the sensitivities of both methods. For SSsc, there is a clear differentiation between the single-cell results (dark blue) versus the negative controls (purple), which represent the inherent experimental noise, providing confidence that the data seen are truly from the sample. However, for the NEB protocol, the background noise, as identified by the negative controls (red), overlaps with the actual single-cell sample (light blue). This clustering reduces confidence in the NEB results, as experimental noise cannot be distinguished from the single-cell sequencing results.

Conclusions  

Extracting meaningful biological information from the small amount of mRNA present in each cell requires an RNA-seq preparation method with exceptional sensitivity and reproducibility. To date, the SMART-Seq v4 kit has been the most sensitive commercial single-cell RNA-seq method, in part due to its incomparable capability to retrieve information from full-length mRNA and not just the 3′ end. To address the need for improvement with extremely challenging samples, such as cells with very low RNA content, we have further modified our core technology to create a new chemistry with higher sensitivity: the SMART-Seq Single Cell Kit. This kit outperforms all current commercial and noncommercial full-length methods, including the NEBNext Single Cell protocol and Smart-seq2, against which our kit shines in terms of convenience, sensitivity, gene identification, and reproducibility. Added benefits are compatibility with automation platforms and a user-friendly plate-based workflow that starts directly from single cells isolated by FACS or other methods. In addition, the SMART-Seq single-cell chemistry generates a high yield of cDNA for repeated and/or alternate modes of analysis, which is extremely useful when dealing with precious and difficult cells such as clinical samples.

Methods  

All cells were labeled with CD81-FITC antibody and 7-AAD (to distinguish live from dead cells) prior to sorting using a BD FACSJazz cell sorter into a 96-well plate or PCR strips. The cell types for each experiment are as noted above and include GM12878, GM22601, PBMCs, or T cells. After sorting, cells were flash frozen on dry ice, and then stored at –80°C until ready to use.

Unless otherwise noted, all libraries created with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing, the SMART-Seq Single Cell Kit, and the NEBNext method were processed at full volume per the user manuals. The only exception to the standard NEBNext protocol was that 25 PCR cycles, versus the standard 20 cycles, were used to produce the NEBNext cDNA libraries. For the comparison to the Smart-seq2 protocol, cells were sorted and processed as described in Picelli et al. 2014.

For all samples except the NEBNext cDNA, sequencing libraries were generated using 125 pg of cDNA and the Nextera® XT DNA Library Preparation Kit (Illumina) with a quarter of the recommended volume, as described in the Nextera XT library prep of cDNA synthesized using the SMART-Seq Single Cell Kit user guide. NEBNext sequencing libraries were processed with 20 ng of NEBNext cDNA (within the recommended range). For all protocol comparisons, the indexed SSsc library and libraries prepared by the alternate methods were pooled together at equal input amounts and purified with Agencourt AMPure XP beads. Libraries were sequenced on a NextSeq® 500 instrument using 2 x 75 bp paired-end reads, and analysis was performed using CLC Genomics Workbench (mapping to the human [hg38] genome with Ensembl annotation). All percentages shown—including the number of reads that map to introns, exons, or intergenic regions—are percentages of mapped reads in each library.

References  

Chenchik, A., Zhu, Y., Diatchenko, L., Li., R., Hill, J. & Siebert, P. Generation and use of high-quality cDNA from small amounts of total RNA by SMART PCR. In RT-PCR Methods for Gene Cloning and Analysis. Eds. Siebert, P. & Larrick, J. (BioTechniques Books, MA), pp. 305–319 (1998).

Hodge, R. D. et al. Conserved cell types with divergent features in human versus mouse cortex. Nature 573, 61–68 (2019).

Ibrayeva, A. et al. Early Stem Cell Aging in the Mature Brain. bioRxiv 654608 (2019).

Picelli, S., Bjorklund, A. K., Faridani, O. R., Sagasser, S., Winberg, G., & Sandberg, R. Smart-seq2 for sensitive full-length transcriptome profiling in single cells. Nat. Methods 10, 1096–1098 (2013).

Picelli, S., Faridani, O. R., Bjorklund, A. K., Winberg, G., Sagasser, S. & Sandberg, R. Full-length RNA-Seq from single cells using Smart-seq2. Nat. Protoc. 9, 171–181 (2014).

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634470 SMART-Seq® Single Cell Kit 12 Rxns USD $1078.00

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The SMART-Seq Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells). This kit supports up to 12 reactions.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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634470: SMART-Seq Single Cell Kit

634470: SMART-Seq Single Cell Kit
634471 SMART-Seq® Single Cell Kit 48 Rxns USD $2982.00

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The SMART-Seq Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells). This kit supports up to 48 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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634471: SMART-Seq Single Cell Kit

634471: SMART-Seq Single Cell Kit
634472 SMART-Seq® Single Cell Kit 96 Rxns USD $5367.00

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The SMART-Seq Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells). This kit supports up to 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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634472: SMART-Seq Single Cell Kit

634472: SMART-Seq Single Cell Kit


Single cell

Want to improve the results of your single-cell RNA-seq experiment?

Successful single-cell RNA-seq doesn't just happen on its own. It takes careful planning and optimization to get quality data you can trust. From sample prep through library prep, we can show you what to expect and how to navigate the difficulties. Explore these general tips for guidance on good experimental practices and FACS tips if you need help with sorting prior to library prep.

General tips FACS tips

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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