RNA-seq
NOTE: We are updating the names and formats of several of our NGS RNA-seq and immune profiling kits. Details of the changes are provided on the relevant product pages for these kits.
See a complete list of RNA-seq kits with new names and the kits they will replace here.
RNA-seq provides unparalleled insight into scientific questions ranging from biological mechanisms to human disease. This technology is enhanced by high-resolution analyses of RNA expression through strand retention and sequencing of both coding and non-coding RNAs. Our SMARTer RNA-Seq kits use our patented SMART (Switching Mechanism At 5' end of RNA Template) technology to provide accurate, highly sensitive, and stranded RNA-seq libraries.
Our systems and their applications are described in the technical notes and other resources below.
mRNA-seq
RNA-seq with UMIs
Robust RNA counting strategy for full-length transcriptome studies from single-cell to 100 ng RNA.
Single-cell RNA-seq
High-quality, full-length RNA-seq libraries from single cells (e.g., PBMCs) and nuclei.
High-throughput RNA-seq
Automated high-quality, full-length RNA-seq library prep from 1–100 cells or 10 pg–1 ng of total RNA.
Ultra-low-input RNA-seq
High-quality, full-length RNA-seq libraries from ultra-low inputs (up to 1,000 cells or 10 pg–10 ng of total RNA).
Ultra-low-input RNA-seq for 3' DE analysis
RNA-seq data that focuses on transcript 3' ends for differential expression analysis.
Targeted RNA-seq
Detect gene fusions and other rare variants using fewer sequencing reads.
Total RNA-seq
Stranded single-cell RNA-seq
High-quality, full-length RNA-seq libraries from single-cells with strand-of-origin information.
Stranded ultra-low-input RNA-seq (mammalian)
Stranded cDNA libraries from 10–1,000 cells or 250 pg–10 ng inputs of mammalian total RNA.
Stranded low-input RNA-seq (mammalian)
Stranded cDNA libraries from 10 ng–100 ng of mammalian total RNA.
Stranded high-input RNA-seq (mammalian)
Stranded cDNA libraries from 100 ng–1 μg of mammalian total RNA.
Stranded RNA-seq from FFPE
Generate stranded libraries from FFPE samples.
Non-stranded RNA-seq from FFPE
Generate non-stranded libraries from FFPE samples.
Sequencer compatibility
Single-cell RNA-seq with the Illumina NextSeq 500
High-quality, full-length RNA-seq libraries from single cells (e.g., PBMCs) and nuclei.
Ultra-low-input RNA-seq with the Illumina NextSeq 500
High-quality, full-length RNA-seq libraries from ultra-low inputs (up to 1,000 cells or 10 pg–10 ng of total RNA).
Single-cell and ultra-low-input RNA-seq with the Singular Genomics G4
Full-length RNA-seq is performed using Takara Bio's SMART-Seq solutions with the Singular Genomics G4 Sequencing Platform.
Automated library prep
View application notes demonstrating automated library prep solutions.
Bioinformatics resources
SMART-Seq DE3 Demultiplexer
Demultiplex sequencing data from SMART-Seq mRNA 3′ DE into sorted read data files.
Cogent NGS Analysis Pipeline
Analyze RNA-seq data generated by select Takara Bio applications.
Cogent NGS Discovery Software
Visualize RNA-seq data in UMAP or t-SNE charts from the output of the Cogent NGS Analysis Pipeline.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
