Gentle and effective primary cell transduction with lentiviral transduction sponge

Primary cells are a powerful tool for molecular biology research, but especially difficult to transduce. Delicate, short-lived, and less permissive to viral transduction, primary cells have historically posed a challenge for researchers using lentiviral tools to establish disease models, conduct genome-wide screens, or study cell-based therapeutic interventions.

This application note assesses the lentiviral transduction efficacy of the Lenti-X Transduction Sponge, compared to other common transduction methods, for primary cultures of hematopoietic stem cells, natural killer cells, and T cells. Read the app note to explore how the Lenti-X Transduction Sponge gently transduces primary cells with greater efficacy than conventional protocols which use spinoculation or chemical enhancers.