- Gene editing
- Viral transduction
- T-cell transduction and culture
- Inducible systems
- Xfect Transfection Reagent
- Transfection tips
- Cell lines transfected with the Xfect reagent
- Protein transfection FAQs
- Xfect RNA Transfection Reagent
- Transfecting with single shots video
- Single shots picture protocol
- Transfection reagent products
- Protocol: transfection of cerebellar slice cultures with Xfect reagent
- Calcium phosphate transfection of neurons
- Fluorescent proteins
Single shots video
State-of-the-art transfection technology
The Xfect reagent is a cationic polymer that binds DNA. It has low cytotoxicity, which means higher cellular viability. Because the Xfect polymer is biodegradable and the transfection protocol doesn't require serum removal, your cells will stay healthier, resulting in a much higher transfection efficiency. Xfect requires minimal reaction optimization: Each Xfect single shots tube comes pre-aliquoted with everything you need to perform a transfection quickly and easily. Just add your DNA!
Figure 1. Xfect transfection reagent yields higher numbers of transfected, viable cells than a popular competitor reagent. Xfect reagent and Product L were each used according to their respective protocols to transfect HeLa cells with increasing amounts of plasmid DNA encoding the Living Colors fluorescent protein, AcGFP1. 48 hr post-transfection, AcGFP1 expression was assessed by flow cytometry in order to determine transfection efficiency, and cell viability was assessed by trypan dye exclusion assay.
Transfection using Xfect Single Shots—picture protocol
Learn more about Xfect transfection
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