- Gene editing
- Viral transduction
- T-cell transduction and culture
- Inducible systems
- Xfect Transfection Reagent
- Transfection tips
- Cell lines transfected with the Xfect reagent
- Protein transfection FAQs
- Xfect RNA Transfection Reagent
- Transfecting with single shots video
- Single shots picture protocol
- Transfection reagent products
- Protocol: transfection of cerebellar slice cultures with Xfect reagent
- Calcium phosphate transfection of neurons
- Fluorescent proteins
- Cell biology assays
Xfect single shots picture protocol
Xfect single shots are pre-aliquoted in individual tubes.
1. Suspend 5 µg of your plasmid containing your gene of interest in 100 µl of water.
|2. Add the diluted plasmid to one of the Xfect single shots.|
|3. Vortex at high speed for 10 sec to mix the sample.
|4. Incubate for 10 min at room temperature to allow DNA and Xfect nanoparticle complexes to form.|
5. Add the entire 100 µl into a single well of a 6-well plate containing 1 ml of complete growth medium. Peak expression is typically reached 48 hr post-transfection.
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