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Home › Learning centers › Automation systems › SmartChip Real-Time PCR System introduction › SmartChip Real-Time PCR System technical specifications

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Technical Specifications

SmartChip Real-Time PCR System technical specifications

The SmartChip system is a complete system that enables high-throughput, high density, real-time PCR for gene expression or single nucleotide polymorphism (SNP) genotyping analysis. The SmartChip MultiSample NanoDispenser (MSND) is a high precision, nanoliter-volume liquid handling system customized with a chip-cooling station and an enclosed humidification environment for delivering multiple samples on custom target sets. In just over 2 hr, the SmartChip Real-Time PCR Cycler can process 5,184 reactions in parallel from a single chip. Gene expression analysis can be performed using dye- or probe-based methods, depending on your research needs. Common SNP genotyping protocols making use of FAM and VIC dyes are optimized to meet your high-throughput experimental needs in a cost-effective manner.

System components SmartChip MSND specifications SmartChip cycler specifications

System components  

SmartChip MSND

  • Load up to 384 samples
  • Active evaporation control
  • Proven high-precision delivery platform

SmartChip MSND

SmartChip cycler

  • 5,184 parallel reactions
  • Approximately 2-hour run time
  • 100-nl reactions

SmartChip Cycler

SmartChip MSND specifications  

Category Specifications
Maximum # of samples 384
Dispense volume 100 nl per well
Laptop computer
  • Enterprise class, 64-bit Windows 7
  • 2-Gb memory
  • 250-Gb storage
  • 1-Gb network and wireless
  • USB port
Power requirements Four outlets (SmartChip MSND, pump control box, CPU, and monitor) with 100–120 VAC/200–240 VAC, 8A/4A at 60/50Hz
Environmental conditions
  • 15–30°C ambient
  • 10–70% relative humidity, noncondensing
Dimensions
  • Fluidic module: (W) 28 cm, (H) 33 cm, (D) 45 cm
  • Stage module: (W) 27 cm, (H) 40 cm, (D) 60 cm
  • Laptop computer: (W) 35 cm, (H) 30 cm, (D) 27 cm
  • Pump control box: (W) 26 cm, (H) 38 cm, (D) 51 cm
  • Humidifier: (W) 20 cm, (H) 40 cm, (D) 30 cm
  • Scale: (W) 25 cm, (H) 50 cm, (D) 22 cm
Weight
  • SmartChip MSND: 65 kg
  • CPU: 2.7 kg
Performance specification Printed lambda, CTSD <0.2
Dispenser run time
  • 48 samples <10 minutes
  • 384 samples <60 minutes
Certifications
  • TUV (North America)
  • CE Mark (European Union)

SmartChip cycler specifications  

Category Specifications
Reactions
  • Parallel reactions: 5,184
  • Reaction volume: 100 nl per well
Precision Standard deviation <0.2 Ct between 5,000 and 10,000 copies of a control template
Filters (center width, nm)
  • Excitation: FAM (475/35), VIC (520/15), ROX (543/22), SYBR (475/42)
  • Emission: FAM (520/15), VIC (565/24), ROX (676/29), SYBR (550/50)
Camera Cooled CCD camera, 9.3 megapixel
Illumination 250 W quartz tungsten halogen stabilized lamp, rated at 1,000 hr
Thermal cycler
  • Ramp rate: 65–95˚C <20 sec, 95–65˚C <15 sec
  • Typical 40-cycle runs take approximately 2 hr
  • Melt temperature profile is user programmable (range: 0.5–2˚C/sec; recommended ramp rate: 0.5˚C/sec)
  • Thermal uniformity is ±0.5˚C at 76˚C
Software SmartChip qPCR instrument control and analysis software. Five user licenses per cycler.
Computer
  • Dell ultra-small form factor
    • Enterprise class, 64-bit Windows 7
    • 4-Gb memory
    • 250-Gb storage
    • 1-Gb network adapter
    • USB ports for memory sticks
  • LCD monitor
Power requirements Two outlets (cycler, CPU and monitor) with 100–120 VAC/200–240 VAC ± 10%, 8.0/4.0 A at 50–60 Hz
Thermal cycler fuse 5 x 20 mm, T6.3AH
Air
  • Clean dry air (recommend −40°C dewpoint) regulated to 6.9–8.3 BAR (100–120 PSI)
  • Regulator output: 4-mm OD urethane tubing or with M5 or 1/8 NPT female port (consult Takara Bio for other thread forms)
Environmental conditions
  • 15–30°C ambient
  • 10–70% relative humidity, noncondensing
  • <2,000 m altitude
  • pollution degree ≤2
Dimensions
  • Thermal cycler: (W) 66 cm, (H) 53 cm, (D) 60 cm
  • CPU, monitor, keyboard, mouse: (W) 60 cm, (H) 50 cm (D) 50 cm
Weight
  • Thermal Cycler unit: 57 kg
  • CPU/monitor: 12 kg
Certifications
  • TUV (North America)
  • CE Mark (European Union)

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