Long-read sequencing is, at times, preferred over traditional short-read methods as it can significantly reduce the complexity of library reconstruction. This technique can be an effective way to improve the detection of fusions, isoforms, and other variants. This guidance protocol demonstrates how the ICELL8's SMART-Seq workflow can be coupled with Oxford Nanopore Technology to support long-read sequencing.
To generate the cDNA library needed for this protocol, please follow the ICELL8 cx 3' DE Reagent Kit User Manual through to the concentration and purification of the library from the chip. STOP before Section V.H (Protocol: Prepare Sequencing Library with the Nextera® XT kit).