LOCUS       prHom-Sec1   7002 bp    DNA   circular            14-FEB-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 635062,635066
FEATURES             Location/Qualifiers
     promoter        19..613
                     /label=CMV\Promoter
     5'UTR           615..655
                     /label=5'\UTR\(HSV\TK)
     CDS             767..1096
                     /label=DmrD
     CDS             1103..1423
                     /label=DmrD
     CDS             1427..1750
                     /label=DmrD
     CDS             1754..2077
                     /label=DmrD
     misc_feature    2081..2104
                     /label=Furin\cleavage\site
     CDS             2105..2677
                     /label=Stuffer\sequence
     misc_feature    692..769
                     /label=Signal\sequence
     3'UTR           2691..3852
                     /label=3'\UTR\(Rabbit\beta-globin\intron\plus\polyA)
     promoter        complement(3861..4056)
                     /label=SV40\promoter
     rep_origin      complement(3939..4016)
                     /label=SV40\origin
     rep_origin      complement(4278..4897)
                     /label=pUC\origin
     CDS             complement(5052..5924)
                     /label=Amp(R)
     rep_origin      complement(6493..6799)
                     /label=f1\origin
BASE COUNT     1759 a      1743 c      1760 g      1740 t 
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg 
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt 
      661 cagggtcctg tggacagctc acctagctgc aatggctaca ggctcccgga cgtccctgct 
      721 cctggctttt ggcctgctct gcctgccctg gcttcaagag ggcagtgcct ctagaggagt 
      781 gcaggtggag actatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg 
      841 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa atggattcct cccgggacag 
      901 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg 
      961 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta 
     1021 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga 
     1081 gcttctaaaa ctggaaacta gaggagtgca ggtggagact atctccccag gagacgggcg 
     1141 caccttcccc aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg 
     1201 aaagaaaatg gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca 
     1261 ggaggtgatc cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa 
     1321 actgactata tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc 
     1381 acatgccact ctcgtcttcg atgtggagct tctaaaactg gaaactagag gagtgcaggt 
     1441 ggaaaccatc tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt 
     1501 gcactacacc gggatgcttg aagatggaaa gaaaatggat tcctcccggg acagaaacaa 
     1561 gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc 
     1621 ccagatgagt gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc 
     1681 cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct 
     1741 aaaactggaa actagaggag tgcaggtgga aaccatctcc ccgggagacg ggcgcacctt 
     1801 ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa 
     1861 aatggattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt 
     1921 gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac 
     1981 tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc 
     2041 cactctcgtc ttcgatgtgg agcttctaaa actggaaact agtgctagaa accgtcagaa 
     2101 gagattccca accattccct taagcagact ttttgacaac gctatgctcc gcgcccatcg 
     2161 tctgcaccag ctggcctttg acacctacca ggagtttgaa gaagcctata tcccaaagga 
     2221 acagaagtat tcattcctgc agaaccccca gacctccctc tgtttctcag agtctattcc 
     2281 gacaccctcc aacagggagg aaacacaaca gaaatccaac ctagagctgc tccgcatctc 
     2341 cctgctgctc atccagtcgt ggctggagcc cgtgcagttc ctcaggagtg tcttcgccaa 
     2401 cagcctggtg tacggcgcct ctgacagcaa cgtctatgac ctcctaaagg acctagagga 
     2461 aggcatccaa acgctgatgg ggaggctgga agatggcagc ccccggactg ggcagatctt 
     2521 caagcagacc tacagcaagt tcgacacaaa ctcacacaac gatgacgcac tactcaagaa 
     2581 ctacgggctg ctctactgct tcaggaagga catggacaag gtcgagacat tcctgcgcat 
     2641 cgtgcagtgc cgctctgtgg agggcagctg tggcttctag cccgggatcc tgagaacttc 
     2701 agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta 
     2761 tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac 
     2821 catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct 
     2881 cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa 
     2941 cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt 
     3001 ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt 
     3061 gttataatta aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat 
     3121 attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc tttatggtta 
     3181 caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct 
     3241 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttgtt 
     3301 gtgctgtctc atcattttgg caaaggattc actcctcagg tgcaggctgc ctatcagaag 
     3361 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct 
     3421 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg 
     3481 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca 
     3541 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca 
     3601 tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa 
     3661 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt 
     3721 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt 
     3781 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct 
     3841 cttatgaaga tccctcgagg agctttttgc aaaagcccta ggcctccaaa aaagcctctt 
     3901 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa 
     3961 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc 
     4021 ggagttaggg gcgggactat ggttgctgac taattgctgc attaatgaat cggccaacgc 
     4081 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 
     4141 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     4201 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     4261 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     4321 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     4381 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     4441 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     4501 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     4561 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     4621 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     4681 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 
     4741 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     4801 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     4861 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     4921 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     4981 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     5041 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     5101 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     5161 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     5221 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     5281 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     5341 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 
     5401 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     5461 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     5521 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     5581 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     5641 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 
     5701 ttaaaagtgc tcatcattgc cacctccacc tgaaaacgtt cttcggggcg aaaactctca 
     5761 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     5821 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     5881 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     5941 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     6001 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     6061 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 
     6121 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 
     6181 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 
     6241 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga 
     6301 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 
     6361 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 
     6421 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 
     6481 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 
     6541 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 
     6601 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 
     6661 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 
     6721 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 
     6781 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc 
     6841 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 
     6901 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 
     6961 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gg 
//
