LOCUS       pZsYellow1-N1   4709 bp    DNA   circular            20-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632445,630050
FEATURES             Location/Qualifiers
     CDS             2605..3399
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     promoter        1..589
                     /label=CMV\IE\Promoter
                     /note="Human cytomegalovirus (CMV) immediate early promoter: Enhancer region: 59-465; TATA box: 554-560;Transcription start point: 583; C->G mutation to remove Sac I site: 569"
     polyA_signal    1528..1578
                     /label=SV40\early\polyA\signal
                     /note="SV40 early mRNA polyadenylation signal Polyadenylation signals: 1552-1557 & 1581-1586; mRNA 3' ends: 1590 & 1602"
     polyA_signal    3635..3653
                     /label=HSV\TK\polyA\signal
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal Polyadenylation signals: 3659-3664 & 3672-3677"
     promoter        2254..2522
                     /label=SV40\early\Promoter
                     /note="SV40 early promoter Enhancer (72-bp tandem repeats): 2278-2349 & 2350-2421; 21-bp repeats: 2425-2445, 2446-2466, & 2468-2488; Early promoter element: 2501-2507; Major transcription start points: 2497, 2535, 2541, & 2546"
     promoter        2142..2177
                     /label=Kan(R)\Promoter
                     /note="Bacterial promoter for expression of Kan R gene; -35 region: 2166-2171; -10 region: 2189-2194; Transcription start point: 2201"
     rep_origin      complement(1625..2080)
                     /label=f1\origin
                     /note="f1 single-strand DNA origin Packages the noncoding strand of EGFP)"
     rep_origin      2421..2556
                     /label=SV40\origin
                     /note="SV40 origin of replication"
     rep_origin      3984..4627
                     /label=pUC\origin
                     /note="pUC plasmid replication origin"
     CDS             679..1371
                     /label=ZsYellow1
     misc_signal     591..671
                     /label=MCS
BASE COUNT     1127 a      1264 c      1256 g      1062 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccaccgg tcgccaccat ggcccacagc aagcacggcc tgaaggagga gatgaccatg 
      721 aagtaccaca tggagggctg cgtgaacggc cacaagttcg tgatcaccgg cgagggcatc 
      781 ggctacccct tcaagggcaa gcagaccatc aacctgtgcg tgatcgaggg cggccccctg 
      841 cccttcagcg aggacatcct gagcgccggc ttcaagtacg gcgaccggat cttcaccgag 
      901 tacccccagg acatcgtgga ctacttcaag aacagctgcc ccgccggcta cacctggggc 
      961 cggagcttcc tgttcgagga cggcgccgtg tgcatctgta acgtggacat caccgtgagc 
     1021 gtgaaggaga actgcatcta ccacaagagc atcttcaacg gcgtgaactt ccccgccgac 
     1081 ggccccgtga tgaagaagat gaccaccaac tgggaggcca gctgcgagaa gatcatgccc 
     1141 gtgcctaagc agggcatcct gaagggcgac gtgagcatgt acctgctgct gaaggacggc 
     1201 ggccggtacc ggtgccagtt cgacaccgtg tacaaggcca agagcgtgcc cagcaagatg 
     1261 cccgagtggc acttcatcca gcacaagctg ctgcgggagg accggagcga cgccaagaac 
     1321 cagaagtggc agctgaccga gcacgccatc gccttcccca gcgccctggc ctgaagcggc 
     1381 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 
     1441 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 
     1501 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     1561 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag 
     1621 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 
     1681 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 
     1741 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 
     1801 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 
     1861 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 
     1921 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 
     1981 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 
     2041 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga 
     2101 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
     2161 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg 
     2221 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 
     2281 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
     2341 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     2401 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     2461 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     2521 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa 
     2581 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 
     2641 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 
     2701 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 
     2761 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg 
     2821 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 
     2881 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 
     2941 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 
     3001 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 
     3061 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 
     3121 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 
     3181 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 
     3241 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 
     3301 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 
     3361 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 
     3421 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 
     3481 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 
     3541 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg 
     3601 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg 
     3661 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata 
     3721 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc 
     3781 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca 
     3841 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
     3901 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     3961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     4021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     4081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     4141 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     4201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     4261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     4321 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     4381 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     4441 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     4501 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     4561 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     4621 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 
     4681 ctgtggataa ccgtattacc gccatgcat 
//
