LOCUS       pZsGreen1-N1   4708 bp    DNA   circular            20-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632448,630050
FEATURES             Location/Qualifiers
     CDS             2604..3398
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     promoter        1..589
                     /label=CMV\IE\Promoter
                     /note="Human cytomegalovirus (CMV) immediate early promoter: Enhancer region: 59-465; TATA box: 554-560;Transcription start point: 583; C->G mutation to remove Sac I site: 569"
     polyA_signal    1527..1577
                     /label=SV40\early\polyA\signal
                     /note="SV40 early mRNA polyadenylation signal Polyadenylation signals: 1552-1557 & 1581-1586; mRNA 3' ends: 1590 & 1602"
     polyA_signal    3634..3652
                     /label=HSV\TK\polyA\signal
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal Polyadenylation signals: 3659-3664 & 3672-3677"
     promoter        2253..2521
                     /label=SV40\\early\Promoter
                     /note="SV40 early promoter Enhancer (72-bp tandem repeats): 2278-2349 & 2350-2421; 21-bp repeats: 2425-2445, 2446-2466, & 2468-2488; Early promoter element: 2501-2507; Major transcription start points: 2497, 2535, 2541, & 2546"
     promoter        2141..2176
                     /label=Kan(R)\Promoter
                     /note="Bacterial promoter for expression of Kan R gene; -35 region: 2166-2171; -10 region: 2189-2194; Transcription start point: 2201"
     rep_origin      complement(1624..2079)
                     /label=f1\origin
                     /note="f1 single-strand DNA origin Packages the noncoding strand of EGFP)"
     rep_origin      2420..2555
                     /label=SV40\origin
                     /note="SV40 origin of replication"
     rep_origin      3983..4626
                     /label=pUC\origin
                     /note="pUC plasmid replication origin"
     CDS             679..1371
                     /label=ZsGreen1
     misc_signal     591..671
                     /label=MCS
BASE COUNT     1110 a      1281 c      1245 g      1072 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccaccgg tcgccaccat ggcccagtcc aagcacggcc tgaccaagga gatgaccatg 
      721 aagtaccgca tggagggctg cgtggacggc cacaagttcg tgatcaccgg cgagggcatc 
      781 ggctacccct tcaagggcaa gcaggccatc aacctgtgcg tggtggaggg cggccccttg 
      841 cccttcgccg aggacatctt gtccgccgcc ttcatgtacg gcaaccgcgt gttcaccgag 
      901 tacccccagg acatcgtcga ctacttcaag aactcctgcc ccgccggcta cacctgggac 
      961 cgctccttcc tgttcgagga cggcgccgtg tgcatctgca acgccgacat caccgtgagc 
     1021 gtggaggaga actgcatgta ccacgagtcc aagttctacg gcgtgaactt ccccgccgac 
     1081 ggccccgtga tgaagaagat gaccgacaac tgggagccct cctgcgagaa gatcatcccc 
     1141 gtgcccaagc agggcatctt gaagggcgac gtgagcatgt acctgctgct gaaggacggt 
     1201 ggccgcttgc gctgccagtt cgacaccgtg tacaaggcca agtccgtgcc ccgcaagatg 
     1261 cccgactggc acttcatcca gcacaagctg acccgcgagg accgcagcga cgccaagaac 
     1321 cagaagtggc acctgaccga gcacgccatc gcctccggct ccgccttgcc ctgagcggcc 
     1381 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 
     1441 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 
     1501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     1561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg 
     1621 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 
     1681 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 
     1741 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 
     1801 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 
     1861 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 
     1921 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 
     1981 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 
     2041 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 
     2101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 
     2161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 
     2221 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 
     2281 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 
     2341 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 
     2401 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 
     2461 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 
     2521 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 
     2581 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 
     2641 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 
     2701 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 
     2761 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 
     2821 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 
     2881 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 
     2941 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 
     3001 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 
     3061 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 
     3121 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 
     3181 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 
     3241 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 
     3301 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 
     3361 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 
     3421 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 
     3481 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 
     3541 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga 
     3601 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg 
     3661 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 
     3721 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 
     3781 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 
     3841 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 
     3901 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 
     3961 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 
     4021 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 
     4081 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 
     4141 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 
     4201 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 
     4261 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 
     4321 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 
     4381 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 
     4441 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 
     4501 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 
     4561 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 
     4621 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 
     4681 tgtggataac cgtattaccg ccatgcat 
//
