LOCUS       pZsGreen1-DR   4251 bp    DNA   circular             9-DEC-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632428
FEATURES             Location/Qualifiers
     CDS             2147..2941
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     polyA_signal    1070..1104
                     /label=SV40\early\polyA\signals
                     /note="SV40 early mRNA polyadenylation signal Polyadenylation signals: 1552-1557 & 1581-1586; mRNA 3' ends: 1590 & 1602"
     polyA_signal    3177..3195
                     /label=HSV\TK\polyA\signals
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal Polyadenylation signals: 3659-3664 & 3672-3677"
     promoter        1794..2025
                     /label=SV40\early\Promoter/Enhancer
                     /note="SV40 early promoter Enhancer (72-bp tandem repeats): 2278-2349 & 2350-2421; 21-bp repeats: 2425-2445, 2446-2466, & 2468-2488; Early promoter element: 2501-2507; Major transcription start points: 2497, 2535, 2541, & 2546"
     promoter        1684..1712
                     /label=Bacterial\Promoter\for\Kan(R)
                     /note="Bacterial promoter for expression of Kan R gene; -35 region: 2166-2171; -10 region: 2189-2194; Transcription start point: 2201"
     rep_origin      complement(1167..1622)
                     /label=f1\origin
                     /note="f1 single-strand DNA origin Packages the noncoding strand of EGFP)"
     rep_origin      1963..2098
                     /label=SV40\origin
                     /note="SV40 origin of replication"
     rep_origin      3526..4169
                     /label=pUC\origin
     CDS             90..914
                     /label=ZsGreen1-DR
     misc_feature    12..83
                     /label=MCS
BASE COUNT      983 a      1175 c      1148 g       945 t 
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg 
       61 acggtaccgc gggcccggga tccgccacca tggcccagtc caagcacggc ctgaccaagg 
      121 agatgaccat gaagtaccgc atggagggct gcgtggacgg ccacaagttc gtgatcaccg 
      181 gcgagggcat cggctacccc ttcaagggca agcaggccat caacctgtgc gtggtggagg 
      241 gcggcccctt gcccttcgcc gaggacatct tgtccgccgc cttcatgtac ggcaaccgcg 
      301 tgttcaccga gtacccccag gacatcgtcg actacttcaa gaactcctgc cccgccggct 
      361 acacctggga ccgctccttc ctgttcgagg acggcgccgt gtgcatctgc aacgccgaca 
      421 tcaccgtgag cgtggaggag aactgcatgt accacgagtc caagttctac ggcgtgaact 
      481 tccccgccga cggccccgtg atgaagaaga tgaccgacaa ctgggagccc tcctgcgaga 
      541 agatcatccc cgtgcccaag cagggcatct tgaagggcga cgtgagcatg tacctgctgc 
      601 tgaaggacgg tggccgcttg cgctgccagt tcgacaccgt gtacaaggcc aagtccgtgc 
      661 cccgcaagat gcccgactgg cacttcatcc agcacaagct gacccgcgag gaccgcagcg 
      721 acgccaagaa ccagaagtgg cacctgaccg agcacgccat cgcctccggc tccgccttgc 
      781 ccaagcttcc gcggagccat ggcttcccgc cggcggtggc ggcgcaggat gatggcacgc 
      841 tgcccatgtc ttgtgcccag gagagcggga tggaccgtca ccctgcagcc tgtgcttctg 
      901 ctaggatcaa tgtgtaggcg gccgcgactc tagatcataa tcagccatac cacatttgta 
      961 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 
     1021 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     1081 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     1141 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc 
     1201 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 
     1261 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 
     1321 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 
     1381 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 
     1441 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 
     1501 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 
     1561 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 
     1621 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 
     1681 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
     1741 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt 
     1801 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 
     1861 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 
     1921 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 
     1981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 
     2041 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 
     2101 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 
     2161 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 
     2221 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 
     2281 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc 
     2341 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 
     2401 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 
     2461 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 
     2521 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 
     2581 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 
     2641 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt 
     2701 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 
     2761 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 
     2821 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 
     2881 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 
     2941 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 
     3001 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 
     3061 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg 
     3121 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata 
     3181 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc 
     3241 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt 
     3301 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac 
     3361 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta 
     3421 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     3481 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     3541 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     3601 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     3661 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 
     3721 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     3781 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     3841 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     3901 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     3961 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     4021 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     4081 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     4141 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 
     4201 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t 
//
