LOCUS       pZsGreen1-C1   4707 bp    DNA   circular            20-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632447,6320050
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label=CMV\IE\Promoter
     rep_origin      2419..2554
                     /label=SV40\origin
     CDS             2603..3395
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     rep_origin      3982..4625
                     /label=pUC\origin
     promoter        2252..2520
                     /label=SV40\early\Promoter
     polyA_signal    1526..1576
                     /label=SV40\early\polyA\signal
     polyA_signal    3633..3651
                     /label=HSV\TK\polyA\signal
     promoter        2140..2175
                     /label=Kan(R)\Promoter
     rep_origin      complement(1623..2078)
                     /label=f1\origin
     CDS             613..1305
                     /label=ZsGreen1
     misc_signal     1315..1393
                     /label=MCS
BASE COUNT     1112 a      1280 c      1241 g      1074 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggccca gtccaagcac ggcctgacca aggagatgac catgaagtac 
      661 cgcatggagg gctgcgtgga cggccacaag ttcgtgatca ccggcgaggg catcggctac 
      721 cccttcaagg gcaagcaggc catcaacctg tgcgtggtgg agggcggccc cttgcccttc 
      781 gccgaggaca tcttgtccgc cgccttcatg tacggcaacc gcgtgttcac cgagtacccc 
      841 caggacatcg tcgactactt caagaactcc tgccccgccg gctacacctg ggaccgctcc 
      901 ttcctgttcg aggacggcgc cgtgtgcatc tgcaacgccg acatcaccgt gagcgtggag 
      961 gagaactgca tgtaccacga gtccaagttc tacggcgtga acttccccgc cgacggcccc 
     1021 gtgatgaaga agatgaccga caactgggag ccctcctgcg agaagatcat ccccgtgccc 
     1081 aagcagggca tcttgaaggg cgacgtgagc atgtacctgc tgctgaagga cggtggccgc 
     1141 ttgcgctgcc agttcgacac cgtgtacaag gccaagtccg tgccccgcaa gatgcccgac 
     1201 tggcacttca tccagcacaa gctgacccgc gaggaccgca gcgacgccaa gaaccagaag 
     1261 tggcacctga ccgagcacgc catcgcctcc ggctccgcct tgccctccgg actcagatct 
     1321 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct 
     1381 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac 
     1441 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 
     1501 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 
     1561 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc 
     1621 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 
     1681 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 
     1741 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 
     1801 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 
     1861 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 
     1921 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 
     1981 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 
     2041 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa 
     2101 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
     2161 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga 
     2221 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 
     2281 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 
     2341 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 
     2401 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 
     2461 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 
     2521 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga 
     2581 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     2641 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     2701 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     2761 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac 
     2821 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     2881 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     2941 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     3001 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     3061 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     3121 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     3181 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     3241 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     3301 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     3361 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 
     3421 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 
     3481 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 
     3541 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag 
     3601 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt 
     3661 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc 
     3721 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc 
     3781 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata 
     3841 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 
     3901 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 
     3961 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 
     4021 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 
     4081 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 
     4141 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 
     4201 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 
     4261 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 
     4321 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 
     4381 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 
     4441 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 
     4501 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 
     4561 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 
     4621 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 
     4681 gtggataacc gtattaccgc catgcat 
//
