LOCUS pUC118 Vector 3162 bp DNA circular SYN 23-JUL-2021 COMMENT Created by Takara Bio Inc. http://www.takarabio.com COMMENT Cat No. 3318 FEATURES Location/Qualifiers promoter 143..173 /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 181..197 /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 217..501 /label="lacZ-alpha" misc_feature 231..287 /label="MCS" /note="pUC18/19 multiple cloning site" primer_bind complement(291..307) /label="M13 fwd" /note="common sequencing primer, one of multiple similar variants" misc_feature 520..975 /label="M13 IG" /note="intergenic region (IG region) of the M13 phage" CDS 1362..2222 /label="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" rep_origin 2393..2981 /direction=RIGHT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcgagc 241 tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc actggccgtc 301 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 361 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 421 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg 481 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat 541 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 601 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 661 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 721 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt 781 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 841 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg 901 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 961 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 1021 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 1081 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 1141 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta 1201 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 1261 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 1321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 1381 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 1441 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 1501 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 1561 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 1621 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 1681 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 1741 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 1801 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 1861 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 1921 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 1981 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 2041 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 2101 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 2161 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 2221 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 2281 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 2341 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 2401 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 2461 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 2521 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 2581 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 2641 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 2701 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 2761 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 2821 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 2881 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 2941 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 3001 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 3061 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 3121 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag //