LOCUS       pTripleEx2   3587 bp    DNA   circular             5-JAN-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 634901
FEATURES             Location/Qualifiers
     misc_feature    94..177
                     /label=lac\Promoter
     misc_feature    276..413
                     /label=5'\UTR
     misc_feature    469..849
                     /label=lacZ\(alpha\fragment)
     misc_feature    398..416
                     /label=ompA\RBS\and\start\codon
     misc_feature    458..471
                     /label=lacZ\RBS\and\start\codon
     misc_feature    529..665
                     /label=MCS
     misc_feature    238..257
                     /label=SP6\RNA\Polymerase\Promoter
     misc_feature    complement(661..680)
                     /label=T7\RNA\Polymerase\Promoter
     misc_feature    508..520
                     /label=Transcription/Translation\Slip\Site
     misc_feature    complement(844..1299)
                     /label=f1\origin
     misc_feature    1357..1390
                     /label=loxP
     misc_feature    1786..2646
                     /label=Amp(R)
     misc_feature    2794..3437
                     /label=pUC\origin
BASE COUNT      922 a       879 c       898 g       888 t 
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctatt 
      241 taggtgacac tatagaatac tcaagctagc tctagcttta catcgcaagg gtgtcggcat 
      301 aagccgaaga tatcggtaga gttatattga gcagatcccc cggtgaagga tttaaccgtg 
      361 ttatctcgtt ggaatattca tggcgtattt tggatgataa cgaggcgcaa aaaatgaaaa 
      421 gtactctagc aattgtgagc ggacaacaat ttcacacagg aaacagctat gaccatgatt 
      481 acgccaagct ccgagatctg gacgagcttt tttttttttt ctcgggaagc gcgccattgt 
      541 gttggtaccc gggaattcgg ccattatggc ctgcaggatc cggccgcctc ggcccagtcg 
      601 actctagact cgagcaagct tatgcatgcg gccgcaattc gagctcactt ggccaattcg 
      661 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa 
      721 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 
      781 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 
      841 tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 
      901 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 
      961 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 
     1021 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 
     1081 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 
     1141 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 
     1201 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 
     1261 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ctgatgcggt attttctcct 
     1321 tacgcatctg tgcggtattt cacaccgcat agatcgataa cttcgtatag catacattat 
     1381 acgaagttat ttaaatcgat atggtgcact ctcagtacaa tctgctctga tgccgcatag 
     1441 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc 
     1501 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt 
     1561 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag 
     1621 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg 
     1681 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 
     1741 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 
     1801 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 
     1861 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 
     1921 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 
     1981 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 
     2041 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 
     2101 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 
     2161 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 
     2221 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 
     2281 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 
     2341 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 
     2401 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 
     2461 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 
     2521 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 
     2581 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 
     2641 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 
     2701 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 
     2761 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     2821 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     2881 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     2941 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 
     3001 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     3061 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     3121 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     3181 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     3241 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     3301 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     3361 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     3421 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 
     3481 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 
     3541 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaga 
//
