LOCUS       pTRE3G-BI-Luc Control                 4516 bp ds-DNA     circular   12-MAR-2020
COMMENT     Created by Takara Bio USA, Inc. http://www.takarabio.com
COMMENT     631332, 631333, 631334, 631337, 631338, 631339, 631340, 631341, 631342, 631343, 631344, 631345, 635079

FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="synthetic DNA construct"
     promoter        1..451
                     /label=TRE3G BI promoter
     CDS             489..2141
                     /label=luciferase
     misc_feature    2159..2346
                     /label=SV40 polyA signal
     rep_origin      2522..3121
                     /label=pUC ori
     CDS             complement(3283..4143)
                     /label=AmpR
     misc_feature    complement(4144..4248)
                     /label=pAmp(R)
     misc_feature    complement(4279..4466)
                     /label=SV40 polyA signal
     misc_feature    4470..4513
                     /label=MCS
ORIGIN
        1 gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccac
       61 ctcgacatac tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
      121 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
      181 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
      241 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
      301 tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga
      361 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc
      421 aactttccgt accacttcct accctcgtaa agtcgacacc ggggcccaga tctccgcggg
      481 gatccaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctag
      541 aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
      601 gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg
      661 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
      721 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
      781 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
      841 tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga
      901 acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct aaaacggatt
      961 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     1021 aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaact
     1081 cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca
     1141 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     1201 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     1261 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc
     1321 aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa
     1381 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac
     1441 ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac
     1501 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     1561 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     1621 ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta
     1681 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     1741 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcata gttgaccgct
     1801 tgaagtcttt aattaaatac aaaggatacc aggtggcccc cgctgaattg gagtcgatat
     1861 tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg
     1921 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     1981 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     2041 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     2101 tcctcataaa ggccaagaag ggcggaaagt ccaaattgta agatatctcc agaggatcat
     2161 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     2221 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     2281 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2341 gccccgagct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2701 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2941 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3361 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3421 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3481 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3541 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3601 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3661 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3721 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3781 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3841 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     3901 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3961 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4021 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4081 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4141 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4201 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4261 aaaagtgcca cctgacgtcg gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     4321 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     4381 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     4441 tacaaatgtg gtatggctga ttatgatcct ctagacatat gctgcagacg cgtggtaccc
     4501 ccggggaatt ctccag
//
