LOCUS       pTRE3G-BI Vector                2880 bp ds-DNA     circular   05-MAR-2020
COMMENT     Created by Takara Bio USA, Inc. http://www.takarabio.com
COMMENT     631332, 631337, 631340, 631343

FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="synthetic DNA construct"
     promoter        1..451
                     /label=TRE3G BI promoter
     misc_feature    461..511
                     /label=MCS-1
     misc_feature    523..710
                     /label=SV40 polyA signal
     rep_origin      886..1485
                     /label=pUC ori
     CDS             complement(1647..2507)
                     /label=AmpR
     misc_feature    complement(2508..2612)
                     /label=pAmp(R)
     misc_feature    complement(2643..2830)
                     /label=SV40 polyA signal
     misc_feature    2834..2877
                     /label=MCS-2
ORIGIN
        1 gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccac
       61 ctcgacatac tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
      121 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
      181 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
      241 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
      301 tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga
      361 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc
      421 aactttccgt accacttcct accctcgtaa agtcgacacc ggggcccaga tctccgcggg
      481 gatccatcga tccgcggccg ccggcgatat ctccagagga tcataatcag ccataccaca
      541 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      601 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      661 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgccccg agcttcctcg
      721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1321 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1921 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1981 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2041 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2101 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2161 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2221 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     2281 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2341 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2401 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2461 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2521 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2581 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2641 gtcggcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2701 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2761 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     2821 ctgattatga tcctctagac atatgctgca gacgcgtggt acccccgggg aattctccag
//
