LOCUS       pTRE-Tight   2605 bp    DNA   circular            13-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 630930,631126,631059,631124,630934
FEATURES             Location/Qualifiers
     misc_feature    3..318
                     /label=Tet-responsive\Promoter
     misc_feature    3..252
                     /label=Modified\Tet-response\element\(TREmod)
     misc_feature    258..317
                     /label=Minimal\CMV\Promoter
     misc_feature    406..606
                     /label=SV40\polyA\signal
     misc_feature    780..1379
                     /label=pUC\origin
     misc_feature    complement(1541..2536)
                     /label=Amp(R)
BASE COUNT      685 a       635 c       603 g       682 t 
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga 
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc 
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg 
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata 
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta 
      301 gtgaaccgtc agatcgcctg gagaattcga gctcggtacc cggggatcct ctagtcagct 
      361 gacgcgtgct agcgcggccg catcgataag cttgtcgacg atatctctag aggatcataa 
      421 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 
      481 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata 
      541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
      601 ctcgagcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
      661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
      721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
      781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
      841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
      901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
      961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     1021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     1081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     1141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     1201 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 
     1261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     1321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     1381 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     1441 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     1501 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     1561 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     1621 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     1681 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     1741 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     1801 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     1861 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     1921 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     1981 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     2041 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     2101 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac 
     2161 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     2221 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     2281 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     2341 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     2401 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     2461 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     2521 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 
     2581 gtatcacgag gccctttcgt cttca 
//
