LOCUS       pTRE-Tight-Luc_DS-IK   4246 bp    DNA   circular             8-OCT-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 630930,631126,631059,631124,631121,631119,631117,631116,631115,631114,630934
FEATURES             Location/Qualifiers
     misc_feature    3..318
                     /label=Tet-responsive\Promoter
     misc_feature    3..252
                     /label=Modified\Tet-response\element\(TREmod)
     misc_feature    258..317
                     /label=Minimal\CMV\Promoter
     misc_feature    2047..2247
                     /label=SV40\polyA\signal
     misc_feature    2421..3020
                     /label=pUC\origin
     misc_feature    complement(3182..4177)
                     /label=Amp(R)
     misc_feature    353..2005
                     /label=Luciferase
BASE COUNT     1163 a       980 c       994 g      1109 t 
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga 
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc 
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg 
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata 
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta 
      301 gtgaaccgtc agatcgcctg gagaattcga gctcggtacc cggggatcca ccatggaaga 
      361 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctagaggatg gaaccgctgg 
      421 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac 
      481 agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt 
      541 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga 
      601 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc 
      661 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc 
      721 taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt 
      781 accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc 
      841 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc 
      901 agagtccttt gatcgtgaca aaacaattgc actgataatg aactcctctg gatctactgg 
      961 gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag 
     1021 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt 
     1081 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt 
     1141 cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca 
     1201 aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa 
     1261 atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt 
     1321 cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac 
     1381 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg 
     1441 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg 
     1501 cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa 
     1561 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat 
     1621 agcttactgg gacgaagacg aacacttctt catagttgac cgcttgaagt ctttaattaa 
     1681 atacaaagga taccaggtgg cccccgctga attggagtcg atattgttac aacaccccaa 
     1741 catcttcgac gcgggcgtgg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc 
     1801 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc 
     1861 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc 
     1921 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa 
     1981 gaagggcgga aagtccaaat tgtaaaatgc tagcgcggcc gcatcgataa gcttgtcgac 
     2041 gatatctcta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa 
     2101 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta 
     2161 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     2221 ataaagcatt tttttcactg cctcgagctt cctcgctcac tgactcgctg cgctcggtcg 
     2281 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     2341 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     2401 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     2461 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     2521 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     2581 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     2641 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     2701 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     2761 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     2821 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 
     2881 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     2941 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     3001 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     3061 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     3121 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     3181 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     3241 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     3301 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     3361 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     3421 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     3481 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     3541 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     3601 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     3661 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     3721 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     3781 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     3841 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     3901 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     3961 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     4021 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     4081 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     4141 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 
     4201 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttca 
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