LOCUS       pTRE-Cycle3   3872 bp    DNA   circular            28-JAN-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631117
FEATURES             Location/Qualifiers
     misc_feature    complement(3804..3872)
                     /label=Minimal\CMV\Promoter\2
     misc_feature    3..252
                     /label=Modified\Tet-Response\Element
     misc_feature    749..936
                     /label=SV40\polyA\signal
     misc_feature    complement(2869..3056)
                     /label=SV40\polyA\signal
     misc_feature    1112..1711
                     /label=pUC\origin
     misc_feature    complement(1873..2868)
                     /label=Amp(R)
     misc_feature    352..675
                     /label=DD
     misc_feature    complement(3091..3786)
                     /label=ZsGreen1
     misc_feature    258..317
                     /label=Minimal\CMV\Promoter\1
BASE COUNT      943 a       974 c       996 g       959 t 
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga 
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc 
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg 
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata 
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta 
      301 gtgaaccgtc agatcgcctg gagatttcga gctcggtacc cggggatcac catgggagtg 
      361 caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgt 
      421 gtggtgcact acaccgggat gcttgaagat ggaaagaaag tcgattcctc ccgggacaga 
      481 aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg 
      541 gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat 
      601 ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag 
      661 cttctaaaac cggaaggatc ctctagtcag ctgacgcgtg ctagcgcggc cgcatcgata 
      721 agcttgtcga cgatatctcc agaggatcat aatcagccat accacatttg tagaggtttt 
      781 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 
      841 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
      901 aaatttcaca aataaagcat ttttttcact gccccgagct tcctcgctca ctgactcgct 
      961 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     1021 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     1081 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     1141 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     1201 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     1261 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
     1321 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     1381 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     1441 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     1501 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 
     1561 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     1621 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     1681 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     1741 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     1801 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     1861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     1921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     1981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     2041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     2101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     2161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     2221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     2281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     2341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     2401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     2461 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 
     2521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     2581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     2641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     2701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     2761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     2821 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg gcagtgaaaa 
     2881 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc 
     2941 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg 
     3001 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct 
     3061 ctagactgca gcctcaggag atctgggccc tcagggcaag gcggagccgg aggcgatggc 
     3121 gtgctcggtc aggtgccact tctggttctt ggcgtcgctg cggtcctcgc gggtcagctt 
     3181 gtgctggatg aagtgccagt cgggcatctt gcggggcacg gacttggcct tgtacacggt 
     3241 gtcgaactgg cagcgcaagc ggccaccgtc cttcagcagc aggtacatgc tcacgtcgcc 
     3301 cttcaagatg ccctgcttgg gcacggggat gatcttctcg caggagggct cccagttgtc 
     3361 ggtcatcttc ttcatcacgg ggccgtcggc ggggaagttc acgccgtaga acttggactc 
     3421 gtggtacatg cagttctcct ccacgctcac ggtgatgtcg gcgttgcaga tgcacacggc 
     3481 gccgtcctcg aacaggaagg agcggtccca ggtgtagccg gcggggcagg agttcttgaa 
     3541 gtagtcgacg atgtcctggg ggtactcggt gaacacgcgg ttgccgtaca tgaaggcggc 
     3601 ggacaagatg tcctcggcga agggcaaggg gccgccctcc accacgcaca ggttgatggc 
     3661 ctgcttgccc ttgaaggggt agccgatgcc ctcgccggtg atcacgaact tgtggccgtc 
     3721 cacgcagccc tccatgcggt acttcatggt catctccttg gtcaggccgt gcttggactg 
     3781 ggccatggtg gcgaattctc caggcgatct gacggttcac taaacgagct ctgcttatat 
     3841 aggcctccca ccgtacacgc cacctcgaca ta 
//
