LOCUS       pSpike D614G Truncated   8535 bp DNA     circular SYN 15-SEP-2021
COMMENT     Created by Takara Bio USA, Inc. http://www.takarabio.com		
COMMENT     Cat. Nos. 631295, 631296
FEATURES             Location/Qualifiers
     promoter        1..768
                     /label="CMV IE promoter"
     intron          768..1417
                     /label="Rabbit beta-globin IVS"
     CDS             1438..5202
                     /label="SARS-CoV-2 Spike D614G Truncated"
                     /note="SARS-CoV-2 spike variant D614G with a 19 amino acid 
                     deletion in the C-terminus"
     polyA_signal    5305..5360
                     /label="beta-globin poly(A) signal"
     misc_feature    complement(6088..6731)
                     /label="pUC origin of replication"
     CDS             complement(6879..7739)
                     /label=AmpR
     promoter        7740..7844
                     /label="AmpR promoter"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcct cgacggatcc gccaccaatg
     1441 ttcgtgtttc tggtgctgct gcccctggtg agctcccagt gcgtgaacct gaccacaagg
     1501 acccagctgc cccctgccta taccaattcc ttcacacggg gcgtgtacta tcccgacaag
     1561 gtgttccgga gcagcgtgct gcacagcaca caggatctgt ttctgccttt cttttccaac
     1621 gtgacctggt tccacgccat ccacgtgagc ggcaccaatg gcacaaagcg gttcgacaat
     1681 ccagtgctgc cctttaacga tggcgtgtac ttcgccagca ccgagaagtc caacatcatc
     1741 agaggctgga tctttggcac cacactggac tctaagacac agagcctgct gatcgtgaac
     1801 aatgccacca acgtggtcat caaggtgtgc gagttccagt tttgtaatga tccattcctg
     1861 ggcgtgtact atcacaagaa caataagtcc tggatggagt ctgagtttcg cgtgtattcc
     1921 tctgccaaca attgtacatt tgagtacgtg tcccagccct tcctgatgga cctggagggc
     1981 aagcagggca atttcaagaa cctgagggag ttcgtgttta agaatatcga tggctacttc
     2041 aaaatctaca gcaagcacac cccaatcaac ctggtgcgcg acctgccaca gggcttctcc
     2101 gccctggagc cactggtgga tctgcccatc ggcatcaaca tcacccggtt tcagacactg
     2161 ctggccctgc acagaagcta cctgacacca ggcgacagct cctctggatg gaccgcagga
     2221 gcagcagcct actatgtggg ctatctgcag cccaggacct tcctgctgaa gtacaacgag
     2281 aatggcacca tcacagacgc cgtggattgc gccctggatc ccctgtccga gaccaagtgt
     2341 acactgaagt cttttaccgt ggagaagggc atctatcaga catctaattt cagggtgcag
     2401 cctaccgaga gcatcgtgcg ctttcccaat atcacaaacc tgtgcccttt tggcgaggtg
     2461 ttcaacgcaa ccaggttcgc aagcgtgtac gcatggaata ggaagcgcat cagcaactgc
     2521 gtggccgact attccgtgct gtacaacagc gcctccttct ctacctttaa gtgctatggc
     2581 gtgtctccca caaagctgaa tgacctgtgc tttaccaacg tgtacgccga tagcttcgtg
     2641 atcaggggcg acgaggtgcg ccagatcgca ccaggacaga caggcaagat cgcagactac
     2701 aattataagc tgcctgacga tttcaccggc tgcgtgatcg cctggaactc caacaatctg
     2761 gattctaaag tgggcggcaa ctacaattat ctgtaccggc tgtttagaaa gtccaatctg
     2821 aagccattcg agagggacat cagcacagaa atctaccagg caggctccac cccatgcaat
     2881 ggagtggagg gctttaactg ttatttccct ctgcagtctt acggcttcca gccaaccaac
     2941 ggcgtgggct atcagcccta ccgcgtggtg gtgctgtcct ttgagctgct gcacgcacct
     3001 gcaacagtgt gcggaccaaa gaagtctacc aatctggtga agaacaagtg cgtgaacttc
     3061 aacttcaacg gactgaccgg cacaggcgtg ctgaccgagt ccaataagaa gttcctgcct
     3121 tttcagcagt tcggcaggga catcgcagat accacagacg ccgtgcgcga ccctcagacc
     3181 ctggagatcc tggatatcac accatgcagc ttcggcggcg tgtccgtgat cacaccaggc
     3241 accaatacaa gcaaccaggt ggccgtgctg tatcagggcg tgaattgtac cgaggtgccc
     3301 gtggcaatcc acgcagatca gctgacccct acatggcggg tgtactctac cggcagcaac
     3361 gtgttccaga caagagcagg atgcctgatc ggagcagagc acgtgaacaa ttcttatgag
     3421 tgcgacatcc ctatcggcgc cggcatctgt gccagctacc agacccagac aaacagccca
     3481 aggagagcac ggtccgtggc aagccagtcc atcatcgcct ataccatgtc tctgggcgcc
     3541 gagaatagcg tggcctactc caacaattct atcgccatcc ctaccaactt cacaatctcc
     3601 gtgaccacag agatcctgcc agtgtctatg accaagacaa gcgtggactg cacaatgtat
     3661 atctgtggcg atagcaccga gtgctccaac ctgctgctgc agtacggcag cttttgtacc
     3721 cagctgaata gagccctgac aggcatcgcc gtggagcagg acaagaacac acaggaggtg
     3781 ttcgcccagg tgaagcaaat ctacaagacc ccacccatca aggactttgg cggcttcaac
     3841 ttcagccaga tcctgcccga tcctagcaag ccctccaagc ggtcttttat cgaggacctg
     3901 ctgttcaaca aggtgaccct ggccgatgcc ggcttcatca agcagtatgg cgattgcctg
     3961 ggcgacatcg ccgccagaga cctgatctgt gcccagaagt ttaatggcct gaccgtgctg
     4021 cctccactgc tgacagatga gatgatcgca cagtacacat ccgccctgct ggcaggcacc
     4081 atcacatctg gatggacctt cggcgcagga gccgccctgc agatcccttt tgccatgcag
     4141 atggcctatc ggttcaacgg catcggcgtg acccagaatg tgctgtacga gaaccagaag
     4201 ctgatcgcca atcagtttaa ctccgccatc ggcaagatcc aggactccct gagctccaca
     4261 gcctctgccc tgggcaagct gcaggatgtg gtgaatcaga acgcccaggc cctgaatacc
     4321 ctggtgaagc agctgtctag caacttcggc gccatctcct ctgtgctgaa tgatatcctg
     4381 agccggctgg acaaggtgga ggcagaggtg cagatcgacc ggctgatcac aggcagactg
     4441 cagagcctgc agacctacgt gacacagcag ctgatcaggg cagcagagat cagggcatcc
     4501 gccaatctgg cagcaaccaa gatgtctgag tgcgtgctgg gccagagcaa gagagtggac
     4561 ttttgtggca agggctatca cctgatgtct ttcccacaga gcgcccctca cggagtggtg
     4621 tttctgcacg tgacctacgt gccagcccag gagaagaact tcaccacagc accagcaatc
     4681 tgccacgatg gcaaggcaca ctttcctagg gagggcgtgt tcgtgtctaa cggcacccac
     4741 tggtttgtga cacagcgcaa tttctacgag ccacagatca tcaccacaga caatacattc
     4801 gtgagcggca actgtgacgt ggtcatcggc atcgtgaaca ataccgtgta tgatcctctg
     4861 cagccagagc tggactcctt taaggaggag ctggataagt acttcaagaa ccacacctct
     4921 cccgacgtgg atctgggcga catctccggc atcaatgcct ctgtggtgaa catccagaag
     4981 gagatcgata ggctcaatga ggttgccaag aatttaaatg aatctctcat cgatctccaa
     5041 gaacttggaa agtatgagca gtatataaaa tggccatggt acatttggct aggttttata
     5101 gctggcttga ttgccatagt aatggtgaca attatgcttt gctgtatgac cagttgctgt
     5161 agttgtctca agggctgttg ttcttgtgga tcctgctgct aatgatggcc aatgccctgg
     5221 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     5281 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     5341 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     5401 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     5461 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     5521 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     5581 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     5641 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacggatcgg
     5701 ccgcaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5761 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5821 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5881 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5941 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6001 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6061 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6121 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6181 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6241 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6301 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6361 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6421 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6481 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6541 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6601 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6661 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6721 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6781 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6841 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6901 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6961 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7021 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7081 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7141 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7201 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7261 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7321 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7381 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7441 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7501 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7561 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7621 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7681 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7741 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7801 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7861 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     7921 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     7981 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     8041 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     8101 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     8161 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     8221 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     8281 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     8341 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     8401 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     8461 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
     8521 agctccaccg cggtg
//
