LOCUS pSpike D614G Truncated 8535 bp DNA circular SYN 15-SEP-2021 COMMENT Created by Takara Bio USA, Inc. http://www.takarabio.com COMMENT Cat. Nos. 631295, 631296 FEATURES Location/Qualifiers promoter 1..768 /label="CMV IE promoter" intron 768..1417 /label="Rabbit beta-globin IVS" CDS 1438..5202 /label="SARS-CoV-2 Spike D614G Truncated" /note="SARS-CoV-2 spike variant D614G with a 19 amino acid deletion in the C-terminus" polyA_signal 5305..5360 /label="beta-globin poly(A) signal" misc_feature complement(6088..6731) /label="pUC origin of replication" CDS complement(6879..7739) /label=AmpR promoter 7740..7844 /label="AmpR promoter" ORIGIN 1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca 61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt 121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat 181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa 241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg 301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc 361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct 421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga 481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa 541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc 601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg 661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac 721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg 781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat 841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct 901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac 961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc 1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct 1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga 1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc 1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt 1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg 1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc 1381 tggttgttgt gctgtctcat cattttggca aagaattcct cgacggatcc gccaccaatg 1441 ttcgtgtttc tggtgctgct gcccctggtg agctcccagt gcgtgaacct gaccacaagg 1501 acccagctgc cccctgccta taccaattcc ttcacacggg gcgtgtacta tcccgacaag 1561 gtgttccgga gcagcgtgct gcacagcaca caggatctgt ttctgccttt cttttccaac 1621 gtgacctggt tccacgccat ccacgtgagc ggcaccaatg gcacaaagcg gttcgacaat 1681 ccagtgctgc cctttaacga tggcgtgtac ttcgccagca ccgagaagtc caacatcatc 1741 agaggctgga tctttggcac cacactggac tctaagacac agagcctgct gatcgtgaac 1801 aatgccacca acgtggtcat caaggtgtgc gagttccagt tttgtaatga tccattcctg 1861 ggcgtgtact atcacaagaa caataagtcc tggatggagt ctgagtttcg cgtgtattcc 1921 tctgccaaca attgtacatt tgagtacgtg tcccagccct tcctgatgga cctggagggc 1981 aagcagggca atttcaagaa cctgagggag ttcgtgttta agaatatcga tggctacttc 2041 aaaatctaca gcaagcacac cccaatcaac ctggtgcgcg acctgccaca gggcttctcc 2101 gccctggagc cactggtgga tctgcccatc ggcatcaaca tcacccggtt tcagacactg 2161 ctggccctgc acagaagcta cctgacacca ggcgacagct cctctggatg gaccgcagga 2221 gcagcagcct actatgtggg ctatctgcag cccaggacct tcctgctgaa gtacaacgag 2281 aatggcacca tcacagacgc cgtggattgc gccctggatc ccctgtccga gaccaagtgt 2341 acactgaagt cttttaccgt ggagaagggc atctatcaga catctaattt cagggtgcag 2401 cctaccgaga gcatcgtgcg ctttcccaat atcacaaacc tgtgcccttt tggcgaggtg 2461 ttcaacgcaa ccaggttcgc aagcgtgtac gcatggaata ggaagcgcat cagcaactgc 2521 gtggccgact attccgtgct gtacaacagc gcctccttct ctacctttaa gtgctatggc 2581 gtgtctccca caaagctgaa tgacctgtgc tttaccaacg tgtacgccga tagcttcgtg 2641 atcaggggcg acgaggtgcg ccagatcgca ccaggacaga caggcaagat cgcagactac 2701 aattataagc tgcctgacga tttcaccggc tgcgtgatcg cctggaactc caacaatctg 2761 gattctaaag tgggcggcaa ctacaattat ctgtaccggc tgtttagaaa gtccaatctg 2821 aagccattcg agagggacat cagcacagaa atctaccagg caggctccac cccatgcaat 2881 ggagtggagg gctttaactg ttatttccct ctgcagtctt acggcttcca gccaaccaac 2941 ggcgtgggct atcagcccta ccgcgtggtg gtgctgtcct ttgagctgct gcacgcacct 3001 gcaacagtgt gcggaccaaa gaagtctacc aatctggtga agaacaagtg cgtgaacttc 3061 aacttcaacg gactgaccgg cacaggcgtg ctgaccgagt ccaataagaa gttcctgcct 3121 tttcagcagt tcggcaggga catcgcagat accacagacg ccgtgcgcga ccctcagacc 3181 ctggagatcc tggatatcac accatgcagc ttcggcggcg tgtccgtgat cacaccaggc 3241 accaatacaa gcaaccaggt ggccgtgctg tatcagggcg tgaattgtac cgaggtgccc 3301 gtggcaatcc acgcagatca gctgacccct acatggcggg tgtactctac cggcagcaac 3361 gtgttccaga caagagcagg atgcctgatc ggagcagagc acgtgaacaa ttcttatgag 3421 tgcgacatcc ctatcggcgc cggcatctgt gccagctacc agacccagac aaacagccca 3481 aggagagcac ggtccgtggc aagccagtcc atcatcgcct ataccatgtc tctgggcgcc 3541 gagaatagcg tggcctactc caacaattct atcgccatcc ctaccaactt cacaatctcc 3601 gtgaccacag agatcctgcc agtgtctatg accaagacaa gcgtggactg cacaatgtat 3661 atctgtggcg atagcaccga gtgctccaac ctgctgctgc agtacggcag cttttgtacc 3721 cagctgaata gagccctgac aggcatcgcc gtggagcagg acaagaacac acaggaggtg 3781 ttcgcccagg tgaagcaaat ctacaagacc ccacccatca aggactttgg cggcttcaac 3841 ttcagccaga tcctgcccga tcctagcaag ccctccaagc ggtcttttat cgaggacctg 3901 ctgttcaaca aggtgaccct ggccgatgcc ggcttcatca agcagtatgg cgattgcctg 3961 ggcgacatcg ccgccagaga cctgatctgt gcccagaagt ttaatggcct gaccgtgctg 4021 cctccactgc tgacagatga gatgatcgca cagtacacat ccgccctgct ggcaggcacc 4081 atcacatctg gatggacctt cggcgcagga gccgccctgc agatcccttt tgccatgcag 4141 atggcctatc ggttcaacgg catcggcgtg acccagaatg tgctgtacga gaaccagaag 4201 ctgatcgcca atcagtttaa ctccgccatc ggcaagatcc aggactccct gagctccaca 4261 gcctctgccc tgggcaagct gcaggatgtg gtgaatcaga acgcccaggc cctgaatacc 4321 ctggtgaagc agctgtctag caacttcggc gccatctcct ctgtgctgaa tgatatcctg 4381 agccggctgg acaaggtgga ggcagaggtg cagatcgacc ggctgatcac aggcagactg 4441 cagagcctgc agacctacgt gacacagcag ctgatcaggg cagcagagat cagggcatcc 4501 gccaatctgg cagcaaccaa gatgtctgag tgcgtgctgg gccagagcaa gagagtggac 4561 ttttgtggca agggctatca cctgatgtct ttcccacaga gcgcccctca cggagtggtg 4621 tttctgcacg tgacctacgt gccagcccag gagaagaact tcaccacagc accagcaatc 4681 tgccacgatg gcaaggcaca ctttcctagg gagggcgtgt tcgtgtctaa cggcacccac 4741 tggtttgtga cacagcgcaa tttctacgag ccacagatca tcaccacaga caatacattc 4801 gtgagcggca actgtgacgt ggtcatcggc atcgtgaaca ataccgtgta tgatcctctg 4861 cagccagagc tggactcctt taaggaggag ctggataagt acttcaagaa ccacacctct 4921 cccgacgtgg atctgggcga catctccggc atcaatgcct ctgtggtgaa catccagaag 4981 gagatcgata ggctcaatga ggttgccaag aatttaaatg aatctctcat cgatctccaa 5041 gaacttggaa agtatgagca gtatataaaa tggccatggt acatttggct aggttttata 5101 gctggcttga ttgccatagt aatggtgaca attatgcttt gctgtatgac cagttgctgt 5161 agttgtctca agggctgttg ttcttgtgga tcctgctgct aatgatggcc aatgccctgg 5221 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc 5281 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt 5341 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc 5401 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca 5461 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat 5521 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt 5581 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct 5641 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacggatcgg 5701 ccgcaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 5761 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 5821 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 5881 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 5941 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 6001 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 6061 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 6121 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6181 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6241 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6301 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6361 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6421 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6481 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6541 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 6601 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 6661 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6721 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 6781 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 6841 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 6901 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 6961 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 7021 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 7081 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 7141 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 7201 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 7261 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 7321 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 7381 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 7441 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 7501 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 7561 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 7621 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 7681 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 7741 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 7801 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 7861 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa 7921 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat 7981 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 8041 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 8101 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta 8161 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 8221 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 8281 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca 8341 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 8401 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 8461 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg 8521 agctccaccg cggtg //