LOCUS       pSpike B.1.351 Truncated   8526 bp DNA     circular SYN 16-SEP-2021
COMMENT	    Created by Takara Bio USA, Inc. http://www.takarabio.com
COMMENT	    Cat. Nos. 631295, 631296
FEATURES             Location/Qualifiers
     promoter        1..768
                     /label="CMVie promoter"
     intron          768..1417
                     /label="Rabbit beta-globin IVS"
     CDS             1438..5193
                     /label="SARS-CoV-2 Spike (B.1.351 truncated)"
                     /note="SARS-Cov-2 spike variant B.1.351 with a 19 amino 
                     acid deletion in the C-terminus"
     polyA_signal    5296..5351
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    complement(6079..6722)
                     /label="pUC origin of replication"
     CDS             complement(6870..7730)
                     /label="AmpR"
     promoter        7731..7835
                     /label="AmpR promoter"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcct cgacggatcc gccaccaatg
     1441 ttcgtgtttc tggtgctgct gcccctggtg agctcccagt gcgtgaactt taccacaagg
     1501 acccagctgc cccctgccta taccaattcc ttcacacggg gcgtgtacta tcccgacaag
     1561 gtgttccgga gcagcgtgct gcacagcaca caggatctgt ttctgccttt cttttccaac
     1621 gtgacctggt tccacgccat ccacgtgagc ggcaccaatg gcacaaagcg gttcgccaat
     1681 ccagtgctgc cctttaacga tggcgtgtac ttcgccagca ccgagaagtc caacatcatc
     1741 agaggctgga tctttggcac cacactggac tctaagacac agagcctgct gatcgtgaac
     1801 aatgccacca acgtggtcat caaggtgtgc gagttccagt tttgtaatga tccattcctg
     1861 ggcgtgtact atcacaagaa caataagtcc tggatggagt ctgagtttcg cgtgtattcc
     1921 tctgccaaca attgtacatt tgagtacgtg tcccagccct tcctgatgga cctggagggc
     1981 aagcagggca atttcaagaa cctgagggag ttcgtgttta agaatatcga tggctacttc
     2041 aaaatctaca gcaagcacac cccaatcaac ctggtgcgcg gcctgccaca gggcttctcc
     2101 gccctggagc cactggtgga tctgcccatc ggcatcaaca tcacccggtt tcagacactg
     2161 cacattagct acctgacacc aggcgacagc tcctctggat ggaccgcagg agcagcagcc
     2221 tactatgtgg gctatctgca gcccaggacc ttcctgctga agtacaacga gaatggcacc
     2281 atcacagacg ccgtggattg cgccctggat cccctgtccg agaccaagtg tacactgaag
     2341 tcttttaccg tggagaaggg catctatcag acatctaatt tcagggtgca gcctaccgag
     2401 agcatcgtgc gctttcccaa tatcacaaac ctgtgccctt ttggcgaggt gttcaacgca
     2461 accaggttcg caagcgtgta cgcatggaat aggaagcgca tcagcaactg cgtggccgac
     2521 tattccgtgc tgtacaacag cgcctccttc tctaccttta agtgctatgg cgtgtctccc
     2581 acaaagctga atgacctgtg ctttaccaac gtgtacgccg atagcttcgt gatcaggggc
     2641 gacgaggtgc gccagatcgc accaggacag acaggcaaca tcgcagacta caattataag
     2701 ctgcctgacg atttcaccgg ctgcgtgatc gcctggaact ccaacaatct ggattctaaa
     2761 gtgggcggca actacaatta tctgtaccgg ctgtttagaa agtccaatct gaagccattc
     2821 gagagggaca tcagcacaga aatctaccag gcaggctcca ccccatgcaa tggagtgaaa
     2881 ggctttaact gttatttccc tctgcagtct tacggcttcc agccaaccta tggcgtgggc
     2941 tatcagccct accgcgtggt ggtgctgtcc tttgagctgc tgcacgcacc tgcaacagtg
     3001 tgcggaccaa agaagtctac caatctggtg aagaacaagt gcgtgaactt caacttcaac
     3061 ggactgaccg gcacaggcgt gctgaccgag tccaataaga agttcctgcc ttttcagcag
     3121 ttcggcaggg acatcgcaga taccacagac gccgtgcgcg accctcagac cctggagatc
     3181 ctggatatca caccatgcag cttcggcggc gtgtccgtga tcacaccagg caccaataca
     3241 agcaaccagg tggccgtgct gtatcagggc gtgaattgta ccgaggtgcc cgtggcaatc
     3301 cacgcagatc agctgacccc tacatggcgg gtgtactcta ccggcagcaa cgtgttccag
     3361 acaagagcag gatgcctgat cggagcagag cacgtgaaca attcttatga gtgcgacatc
     3421 cctatcggcg ccggcatctg tgccagctac cagacccaga caaacagccc aaggagagca
     3481 cggtccgtgg caagccagtc catcatcgcc tataccatgt ctctgggcgt ggagaatagc
     3541 gtggcctact ccaacaattc tatcgccatc cctaccaact tcacaatctc cgtgaccaca
     3601 gagatcctgc cagtgtctat gaccaagaca agcgtggact gcacaatgta tatctgtggc
     3661 gatagcaccg agtgctccaa cctgctgctg cagtacggca gcttttgtac ccagctgaat
     3721 agagccctga caggcatcgc cgtggagcag gacaagaaca cacaggaggt gttcgcccag
     3781 gtgaagcaaa tctacaagac cccacccatc aaggactttg gcggcttcaa cttcagccag
     3841 atcctgcccg atcctagcaa gccctccaag cggtctttta tcgaggacct gctgttcaac
     3901 aaggtgaccc tggccgatgc cggcttcatc aagcagtatg gcgattgcct gggcgacatc
     3961 gccgccagag acctgatctg tgcccagaag tttaatggcc tgaccgtgct gcctccactg
     4021 ctgacagatg agatgatcgc acagtacaca tccgccctgc tggcaggcac catcacatct
     4081 ggatggacct tcggcgcagg agccgccctg cagatccctt ttgccatgca gatggcctat
     4141 cggttcaacg gcatcggcgt gacccagaat gtgctgtacg agaaccagaa gctgatcgcc
     4201 aatcagttta actccgccat cggcaagatc caggactccc tgagctccac agcctctgcc
     4261 ctgggcaagc tgcaggatgt ggtgaatcag aacgcccagg ccctgaatac cctggtgaag
     4321 cagctgtcta gcaacttcgg cgccatctcc tctgtgctga atgatatcct gagccggctg
     4381 gacaaggtgg aggcagaggt gcagatcgac cggctgatca caggcagact gcagagcctg
     4441 cagacctacg tgacacagca gctgatcagg gcagcagaga tcagggcatc cgccaatctg
     4501 gcagcaacca agatgtctga gtgcgtgctg ggccagagca agagagtgga cttttgtggc
     4561 aagggctatc acctgatgtc tttcccacag agcgcccctc acggagtggt gtttctgcac
     4621 gtgacctacg tgccagccca ggagaagaac ttcaccacag caccagcaat ctgccacgat
     4681 ggcaaggcac actttcctag ggagggcgtg ttcgtgtcta acggcaccca ctggtttgtg
     4741 acacagcgca atttctacga gccacagatc atcaccacag acaatacatt cgtgagcggc
     4801 aactgtgacg tggtcatcgg catcgtgaac aataccgtgt atgatcctct gcagccagag
     4861 ctggactcct ttaaggagga gctggataag tacttcaaga accacacctc tcccgacgtg
     4921 gatctgggcg acatctccgg catcaatgcc tctgtggtga acatccagaa ggagatcgat
     4981 aggctcaatg aggttgccaa gaatttaaat gaatctctca tcgatctcca agaacttgga
     5041 aagtatgagc agtatataaa atggccatgg tacatttggc taggttttat agctggcttg
     5101 attgccatag taatggtgac aattatgctt tgctgtatga ccagttgctg tagttgtctc
     5161 aagggctgtt gttcttgtgg atcctgctgc taatgatggc caatgccctg gctcacaaat
     5221 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     5281 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     5341 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     5401 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     5461 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     5521 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     5581 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     5641 tcccagtcat agctgtccct cttctcttat ggagatccct cgacggatcg gccgcaattc
     5701 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5761 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5821 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5881 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5941 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6001 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6061 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6121 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6181 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6241 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6301 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6361 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6421 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6481 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6541 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6601 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6661 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6721 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6781 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6841 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6901 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6961 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7021 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7081 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7141 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7201 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7261 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7321 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7381 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7441 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7501 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7561 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7621 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7681 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7741 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7801 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7861 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     7921 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     7981 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     8041 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     8101 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     8161 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     8221 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     8281 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     8341 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     8401 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     8461 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg gagctccacc
     8521 gcggtg
//
