LOCUS       pSpike B.1.1.7 Truncated   8540 bp DNA     circular SYN 16-SEP-2021
COMMENT     Created by Takara Bio USA, Inc. http://www.takarabio.com
COMMENT	    Cat. Nos. 631295, 631296
FEATURES             Location/Qualifiers
     promoter        1..768
                     /label="CMVie promoter"
     intron          768..1417
                     /label="Rabbit beta-globin IVS"
     CDS             1445..5197
                     /label="Spike protein (B.1.1.7 truncated)"
                     /note="SARS-Cov-2 spike variant B.1.1.7 with a 19 amino 
                     acid deletion in the C-terminus"
     polyA_signal    5310..5365
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    complement(6093..6736)
                     /label="pUC origin of replication"
     gene            complement(6884..7744)
                     /label="AmpR"
     promoter        7745..7849
                     /label="AmpR promoter"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcct cgacggatcc gccaccaagc
     1441 taccatgttc gtgtttctgg tgctgctgcc cctggtgagc tcccagtgcg tgaacctgac
     1501 cacaaggacc cagctgcccc ctgcctatac caattccttc acacggggcg tgtactatcc
     1561 cgacaaggtg ttccggagca gcgtgctgca cagcacacag gatctgtttc tgcctttctt
     1621 ttccaacgtg acctggttcc acgccatcag cggcaccaat ggcacaaagc ggttcgacaa
     1681 tccagtgctg ccctttaacg atggcgtgta cttcgccagc accgagaagt ccaacatcat
     1741 cagaggctgg atctttggca ccacactgga ctctaagaca cagagcctgc tgatcgtgaa
     1801 caatgccacc aacgtggtca tcaaggtgtg cgagttccag ttttgtaatg atccattcct
     1861 gggcgtgtat cacaagaaca ataagtcctg gatggagtct gagtttcgcg tgtattcctc
     1921 tgccaacaat tgtacatttg agtacgtgtc ccagcccttc ctgatggacc tggagggcaa
     1981 gcagggcaat ttcaagaacc tgagggagtt cgtgtttaag aatatcgatg gctacttcaa
     2041 aatctacagc aagcacaccc caatcaacct ggtgcgcgac ctgccacagg gcttctccgc
     2101 cctggagcca ctggtggatc tgcccatcgg catcaacatc acccggtttc agacactgct
     2161 ggccctgcac agaagctacc tgacaccagg cgacagctcc tctggatgga ccgcaggagc
     2221 agcagcctac tatgtgggct atctgcagcc caggaccttc ctgctgaagt acaacgagaa
     2281 tggcaccatc acagacgccg tggattgcgc cctggatccc ctgtccgaga ccaagtgtac
     2341 actgaagtct tttaccgtgg agaagggcat ctatcagaca tctaatttca gggtgcagcc
     2401 taccgagagc atcgtgcgct ttcccaatat cacaaacctg tgcccttttg gcgaggtgtt
     2461 caacgcaacc aggttcgcaa gcgtgtacgc atggaatagg aagcgcatca gcaactgcgt
     2521 ggccgactat tccgtgctgt acaacagcgc ctccttctct acctttaagt gctatggcgt
     2581 gtctcccaca aagctgaatg acctgtgctt taccaacgtg tacgccgata gcttcgtgat
     2641 caggggcgac gaggtgcgcc agatcgcacc aggacagaca ggcaagatcg cagactacaa
     2701 ttataagctg cctgacgatt tcaccggctg cgtgatcgcc tggaactcca acaatctgga
     2761 ttctaaagtg ggcggcaact acaattatct gtaccggctg tttagaaagt ccaatctgaa
     2821 gccattcgag agggacatca gcacagaaat ctaccaggca ggctccaccc catgcaatgg
     2881 agtggagggc tttaactgtt atttccctct gcagtcttac ggcttccagc caacctatgg
     2941 cgtgggctat cagccctacc gcgtggtggt gctgtccttt gagctgctgc acgcacctgc
     3001 aacagtgtgc ggaccaaaga agtctaccaa tctggtgaag aacaagtgcg tgaacttcaa
     3061 cttcaacgga ctgaccggca caggcgtgct gaccgagtcc aataagaagt tcctgccttt
     3121 tcagcagttc ggcagggaca tcgatgatac cacagacgcc gtgcgcgacc ctcagaccct
     3181 ggagatcctg gatatcacac catgcagctt cggcggcgtg tccgtgatca caccaggcac
     3241 caatacaagc aaccaggtgg ccgtgctgta tcagggcgtg aattgtaccg aggtgcccgt
     3301 ggcaatccac gcagatcagc tgacccctac atggcgggtg tactctaccg gcagcaacgt
     3361 gttccagaca agagcaggat gcctgatcgg agcagagcac gtgaacaatt cttatgagtg
     3421 cgacatccct atcggcgccg gcatctgtgc cagctaccag acccagacaa acagccatag
     3481 gagagcacgg tccgtggcaa gccagtccat catcgcctat accatgtctc tgggcgccga
     3541 gaatagcgtg gcctactcca acaattctat cgccatccct attaacttca caatctccgt
     3601 gaccacagag atcctgccag tgtctatgac caagacaagc gtggactgca caatgtatat
     3661 ctgtggcgat agcaccgagt gctccaacct gctgctgcag tacggcagct tttgtaccca
     3721 gctgaataga gccctgacag gcatcgccgt ggagcaggac aagaacacac aggaggtgtt
     3781 cgcccaggtg aagcaaatct acaagacccc acccatcaag gactttggcg gcttcaactt
     3841 cagccagatc ctgcccgatc ctagcaagcc ctccaagcgg tcttttatcg aggacctgct
     3901 gttcaacaag gtgaccctgg ccgatgccgg cttcatcaag cagtatggcg attgcctggg
     3961 cgacatcgcc gccagagacc tgatctgtgc ccagaagttt aatggcctga ccgtgctgcc
     4021 tccactgctg acagatgaga tgatcgcaca gtacacatcc gccctgctgg caggcaccat
     4081 cacatctgga tggaccttcg gcgcaggagc cgccctgcag atcccttttg ccatgcagat
     4141 ggcctatcgg ttcaacggca tcggcgtgac ccagaatgtg ctgtacgaga accagaagct
     4201 gatcgccaat cagtttaact ccgccatcgg caagatccag gactccctga gctccacagc
     4261 ctctgccctg ggcaagctgc aggatgtggt gaatcagaac gcccaggccc tgaataccct
     4321 ggtgaagcag ctgtctagca acttcggcgc catctcctct gtgctgaatg atatcctggc
     4381 gcggctggac aaggtggagg cagaggtgca gatcgaccgg ctgatcacag gcagactgca
     4441 gagcctgcag acctacgtga cacagcagct gatcagggca gcagagatca gggcatccgc
     4501 caatctggca gcaaccaaga tgtctgagtg cgtgctgggc cagagcaaga gagtggactt
     4561 ttgtggcaag ggctatcacc tgatgtcttt cccacagagc gcccctcacg gagtggtgtt
     4621 tctgcacgtg acctacgtgc cagcccagga gaagaacttc accacagcac cagcaatctg
     4681 ccacgatggc aaggcacact ttcctaggga gggcgtgttc gtgtctaacg gcacccactg
     4741 gtttgtgaca cagcgcaatt tctacgagcc acagatcatc accacacata atacattcgt
     4801 gagcggcaac tgtgacgtgg tcatcggcat cgtgaacaat accgtgtatg atcctctgca
     4861 gccagagctg gactccttta aggaggagct ggataagtac ttcaagaacc acacctctcc
     4921 cgacgtggat ctgggcgaca tctccggcat caatgcctct gtggtgaaca tccagaagga
     4981 gatcgatagg ctcaatgagg ttgccaagaa tttaaatgaa tctctcatcg atctccaaga
     5041 acttggaaag tatgagcagt atataaaatg gccatggtac atttggctag gttttatagc
     5101 tggcttgatt gccatagtaa tggtgacaat tatgctttgc tgtatgacca gttgctgtag
     5161 ttgtctcaag ggctgttgtt cttgtggatc ctgctgctga aactaactaa gtaacaatgc
     5221 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     5281 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     5341 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     5401 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     5461 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     5521 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     5581 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     5641 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacgg
     5701 atcggccgca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5761 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5821 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5881 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5941 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6001 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6061 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6541 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7201 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7261 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7321 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7381 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7441 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7501 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7561 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7621 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7681 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7741 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7801 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7861 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     7921 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     7981 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     8041 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     8101 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     8161 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     8221 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     8281 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     8341 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     8401 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     8461 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     8521 attggagctc caccgcggtg
//
