LOCUS       pRetroX-TetOne-Puro Vector                9464 bp ds-DNA     circular SYN 09-MAR-2020
DEFINITION  synthetic circular DNA
COMMENT    Created by Takara Bio USA, Inc. http://www.takarabio.com
COMMENT    634307, 634308, 634309

FEATURES             Location/Qualifiers
     source          1..9464
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             375..910
                     /label=CMV/MLV 5'LTR
     misc_feature    973..1330
                     /label=MMLV Psi
     polyA_signal    2297..2431
                     /label=SV40 poly(A) signal
     misc_feature    2606..2637
                     /label=MCS
     promoter        complement(2638..3002)
                     /label=TRE3GS Promoter
     promoter        3022..3532
                     /label=hPGK promoter
     CDS             3551..4297
                     /label=Tet-On(R) 3G
     promoter        4315..4644
                     /label=SV40 promoter
     CDS             4653..5252
                     /label=PuroR
     misc_feature    5269..5672
                     /label=WPRE
     LTR             5802..6049
                     /label=3'-LTR
     promoter        7326..7430
                     /gene="bla"
     CDS             7431..8291
                     /label=AmpR
     rep_origin      8462..9050
                     /label=ori

ORIGIN
        1 aagctagctt tgctcttagg agtttcctaa tacatcccaa actcaaatat ataaagcatt
       61 tgacttgttc tatgccctag gagcttggcc cattgcatac gttgtatcca tatcataata
      121 tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta
      181 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      241 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      301 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      361 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      421 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      481 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      541 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      601 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      661 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcatc tagagcgctt
      721 ctgctccccg agctctataa aagagcccac aacccctcac tcggggcgcc agtcctccga
      781 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
      841 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
      901 gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca
      961 ccaccgggag gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat
     1021 gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac
     1081 ccgtggtgga actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga
     1141 cttcgggggc cgtttttgtg gcccgacctg agtcctaaaa tcccgatcgt ttaggactct
     1201 ttggtgcacc ccccttagag gagggatatg tggttctggt aggagacgag aacctaaaac
     1261 agttcccgcc tccgtctgaa tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc
     1321 ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg
     1381 aaaatgaggg cccgggctag cctgttacca ctcccttaag tttgacctta ggtcactgga
     1441 aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta
     1501 ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta
     1561 accgagacct catcacccag gttaagatca aggtcttttc acctggcccg catggacacc
     1621 cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg
     1681 tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc
     1741 cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt
     1801 ctctaggcgc ccccatatgg ccatatgaga tcctatatgg ggcacccccg ccccttgtaa
     1861 acttccctga ccctgacatg acaagagtta ctaacagccc ctctctccaa gctcacttac
     1921 aggctctcta cttagtccag cacgaagtct ggagacctct ggcggcagcc taccaagaac
     1981 aactggaccg accggtggta cctcaccctt accgagtcgg cgacacagtg tgggtccgcc
     2041 gacaccagac taagaaccta gaacctcgct ggaaaggacc ttacacagtc ctgctgacca
     2101 cccccaccgc cctcaaagta gacggcatcg cagcttggat acacgccgcc cacgtgaagg
     2161 ctgccgaccc cgggggtgga ccatcctcta gatcataatc agccatacca catttgtaga
     2221 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2281 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaggcaatag
     2341 catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg gtttgtccaa
     2401 actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg cgcctgtctt
     2461 aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca gtagctctga
     2521 cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat tggctgtcca
     2581 gcttagctcg caggggaggt ggtctggatc cgccggcacc ggtgtatacg ggaattcttt
     2641 acgagggtag gaagtggtac ggaaagttgg tataagacaa aagtgttgtg gaattgaagt
     2701 ttactcaaaa aatcagcact cttttatagg cgccctggtt tacataagca aagcttatac
     2761 gttctctatc actgataggg agtaaactgg atatacgttc tctatcactg atagggagta
     2821 aactgtagat acgttctcta tcactgatag ggagtaaact ggtcatacgt tctctatcac
     2881 tgatagggag taaactcctt atacgttctc tatcactgat agggagtaaa gtctgcatac
     2941 gttctctatc actgataggg agtaaactct tcatacgttc tctatcactg atagggagta
     3001 aactcgaggt gataattcca cggggttggg gttgcgcctt ttccaaggca gccctgggtt
     3061 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     3121 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     3181 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     3241 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     3301 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     3361 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     3421 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     3481 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatca
     3541 tcgaattacc atgtctagac tggacaagag caaagtcata aactctgctc tggaattact
     3601 caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
     3661 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
     3721 cgagatgctg gacaggcatc atacccactc ctgccccctg gaaggcgagt catggcaaga
     3781 ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc
     3841 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
     3901 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
     3961 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
     4021 aagagagaca cctaccaccg attctatgcc cccacttctg aaacaagcaa ttgagctgtt
     4081 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct
     4141 ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt
     4201 agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc
     4261 tcttgacgat tttgaccttg acatgctccc cgggtaacta agtaagaatt gaatgtgtgt
     4321 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4381 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4441 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4501 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4561 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4621 tttggaggcc taggcttttg caaaacgcga ccatgaccga gtacaagccc acggtgcgcc
     4681 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     4741 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     4801 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     4861 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     4921 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     4981 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     5041 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     5101 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     5161 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     5221 cctggtgcat gacccgcaag cccggtgcct gaacgcgtaa ttcctgttaa tcaacctctg
     5281 gattacaaaa tttgtgaaag attgactgat attcttaact atgttgctcc ttttacgctg
     5341 tgtggatatg ctgctttaat gcctctgtat catgctattg cttcccgtac ggctttcgtt
     5401 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5461 cgtcaacgtg gcgtggtgtg ctctgtgttt gctgacgcaa cccccactgg ctggggcatt
     5521 gccaccacct gtcaactcct ttctgggact ttcgctttcc ccctccctat cgccacggca
     5581 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctaggttgct gggcactgat
     5641 aattccgtgg tgttgtcggg gaagctgacg tcgagatctt cctgcggaga tatcagtggt
     5701 ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata
     5761 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     5821 taggtttggc aagctagctt ctcgcttctg ttcgcgcgct tctgctccca gacatcaaca
     5881 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     5941 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     6001 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac gcgtatgccg
     6061 catagttaag ccaccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6121 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6181 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6241 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6301 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6361 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6421 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     6481 cagcgcccga ccgaaaggag cgcacgaccc catggctccg accgaagcca cccggggcgg
     6541 ccccgccgac cccgcacccg cccccgaggc ccaccgactc tagaggatca taatcagcca
     6601 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     6661 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     6721 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6781 ttgtggtttg tccaaactca tcaatgtatc ttatcatggc gcgtcgaatt aattcactgg
     6841 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     6901 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6961 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     7021 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     7081 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7141 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     7201 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     7261 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     7321 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7381 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7441 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7501 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7561 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7621 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7681 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7741 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7801 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7861 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7921 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7981 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8041 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8101 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8161 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8221 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8281 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8341 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8401 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8461 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8521 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8581 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8641 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8701 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8761 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8821 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8881 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8941 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9001 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9061 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9241 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9301 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     9361 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     9421 gataacaatt tcacacagga aacagctatg accatgatta cgcc
//
