LOCUS       pRetroX-SG2Mcyto-Red   7737 bp    DNA   circular            24-SEP-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631464
FEATURES             Location/Qualifiers
     LTR             1..727
                     /label=5'LTR\(CMV/MSV)
     promoter        1582..2170
                     /label=CMV\IE\Promoter
     CDS             2194..3111
                     /label=mCherry-hGeminin(1-60)
     misc_feature    3123..3631
                     /label=PGK\Promoter
     CDS             3652..4251
                     /label=Puro(R)
     misc_feature    4694..4709
                     /label=PolyA\signal
     misc_feature    5148..5415
                     /label=SV40\Promoter
     rep_origin      5369..5434
                     /label=SV40\origin
     CDS             6515..7375
                     /label=Amp(R)
     misc_feature    757..1566
                     /label=PSI+\(extended\packaging\signal)
     LTR             4436..4868
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     rep_origin      5754..6353
                     /label=pUC\origin
BASE COUNT     1767 a      2200 c      2029 g      1741 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca 
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa 
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac 
     2221 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 
     2281 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 
     2341 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 
     2401 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 
     2461 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 
     2521 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 
     2581 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 
     2641 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 
     2701 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 
     2761 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 
     2821 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 
     2881 ggcatggacg agctgtacaa gggatatcca tcacactggc ggccgctcga gatgaatccc 
     2941 agtatgaagc agaaacaaga agaaatcaaa gagaatataa agaatagttc tgtcccaaga 
     3001 agaactctga agatgattca gccttctgca tctggatctc ttgttggaag agaaaatgag 
     3061 ctgtccgcag gcttgtccaa aaggaaacat cggaatgacc acttaacatc ttaactgatc 
     3121 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 
     3181 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 
     3241 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 
     3301 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 
     3361 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 
     3421 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 
     3481 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc 
     3541 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 
     3601 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag 
     3661 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 
     3721 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 
     3781 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 
     3841 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 
     3901 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 
     3961 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 
     4021 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 
     4081 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 
     4141 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 
     4201 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc 
     4261 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcggc gtctcgatcg 
     4321 agatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag 
     4381 agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga 
     4441 aagaccccac ctgtaggttt ggcaagctac tagcttaagt aacgccattt tgcaaggcat 
     4501 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag 
     4561 ggtcgatcga ccctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga 
     4621 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct 
     4681 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt 
     4741 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg 
     4801 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt 
     4861 ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc 
     4921 accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 
     4981 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 
     5041 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 
     5101 agcgatagcg gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc 
     5161 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 
     5221 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 
     5281 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 
     5341 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 
     5401 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 
     5461 aaaaagctta ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 
     5521 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg 
     5581 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     5641 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     5701 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     5761 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     5821 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     5881 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     5941 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     6001 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     6061 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     6121 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     6181 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     6241 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     6301 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     6361 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     6421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     6481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     6541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     6601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     6661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     6721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     6781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg 
     6841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     6901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     6961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     7021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     7081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata 
     7141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     7201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     7261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     7321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     7381 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     7441 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     7501 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 
     7561 cgaggccctt tcgtcttcaa gaattagctt ggccattgca tacgttgtat ccatatcata 
     7621 atatgtacat ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga 
     7681 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagt 
//
