LOCUS       pRetroX-SG2M-Red   7887 bp    DNA   circular            16-SEP-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631465
FEATURES             Location/Qualifiers
     LTR             1..727
                     /label=5'\LTR\(CMV/MSV)
     misc_feature    757..1566
                     /label=PSI+\(extended\packaging\signal)
     misc_feature    1582..2170
                     /label=CMV\IE\Promoter
     CDS             2194..3261
                     /label=mCherry-hGeminin(1-110)
     misc_feature    3273..3781
                     /label=PGK\Promoter
     CDS             3802..4401
                     /label=Puro(R)
     LTR             4586..5018
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     misc_feature    4844..4859
                     /label=PolyA\signal
     misc_feature    5298..5565
                     /label=SV40\Promoter
     rep_origin      5519..5584
                     /label=SV40\origin
     CDS             complement(6665..7525)
                     /label=Amp(R)
     rep_origin      5904..6503
                     /label=pUC\origin
     misc_feature    3262..3264
                     /label=Stop\codon
BASE COUNT     1818 a      2228 c      2065 g      1776 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca 
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa 
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac 
     2221 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 
     2281 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 
     2341 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 
     2401 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 
     2461 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 
     2521 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 
     2581 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 
     2641 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 
     2701 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 
     2761 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 
     2821 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 
     2881 ggcatggacg agctgtacaa gggatatcca tcacactggc ggccgctcga gatgaatccc 
     2941 agtatgaagc agaaacaaga agaaatcaaa gagaatataa agaatagttc tgtcccaaga 
     3001 agaactctga agatgattca gccttctgca tctggatctc ttgttggaag agaaaatgag 
     3061 ctgtccgcag gcttgtccaa aaggaaacat cggaatgacc acttaacatc tacaacttcc 
     3121 agccctgggg ttattgtccc agaatctagt gaaaataaaa atcttggagg agtcacccag 
     3181 gagtcatttg atcttatgat taaagaaaat ccatcctctc agtattggaa ggaagtggca 
     3241 gaaaaacgga gaaaggcgct gtaactgatc ataattctac cgggtagggg aggcgctttt 
     3301 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag 
     3361 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt 
     3421 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc 
     3481 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca 
     3541 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca 
     3601 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct 
     3661 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg 
     3721 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc 
     3781 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc 
     3841 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 
     3901 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 
     3961 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 
     4021 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 
     4081 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 
     4141 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 
     4201 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 
     4261 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 
     4321 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 
     4381 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc 
     4441 gcacgacccc atgcatcggc gtctcgatcg agatatcagt ggtccaggct ctagttttga 
     4501 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt 
     4561 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctac 
     4621 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt 
     4681 tcagatcaag gtcaggaaca gatggaacag ggtcgatcga ccctagagaa ccatcagatg 
     4741 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag 
     4801 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa 
     4861 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca 
     4921 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct 
     4981 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg 
     5041 agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt 
     5101 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 
     5161 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 
     5221 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtagat ccggctgtgg 
     5281 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 
     5341 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 
     5401 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 
     5461 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 
     5521 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 
     5581 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ctggcttaac tatgcggcat 
     5641 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 
     5701 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     5761 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     5821 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     5881 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     5941 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     6001 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     6061 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     6121 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     6181 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     6241 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     6301 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
     6361 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     6421 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     6481 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     6541 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     6601 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     6661 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     6721 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     6781 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     6841 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     6901 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     6961 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     7021 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     7081 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     7141 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     7201 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     7261 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag 
     7321 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     7381 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     7441 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     7501 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     7561 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     7621 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 
     7681 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattagctt 
     7741 ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa 
     7801 cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt 
     7861 cattagttca tagcccatat atggagt 
//
