LOCUS       pRetroX-G1-Red   7830 bp    DNA   circular            24-SEP-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631463
FEATURES             Location/Qualifiers
     CDS             2194..3204
                     /label=mCherry-hCdt1(30-120)
     LTR             1..727
                     /label=5'\LTR\(CMV/MSV)
     misc_feature    757..1566
                     /label=PSI+\(extended\packaging\signal)
     promoter        1582..2170
                     /label=CMV\IE\Promoter
     promoter        3216..3724
                     /label=PGK\Promoter
     CDS             3745..4344
                     /label=Puro(R)
     LTR             4529..4961
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     polyA_signal    4787..4802
                     /label=PolyA\signal
     promoter        5241..5508
                     /label=SV40\Promoter
     rep_origin      5462..5527
                     /label=SV40\origin
     CDS             complement(6608..7468)
                     /label=Amp(R)
     rep_origin      5847..6446
                     /label=pUC\origin
     misc_feature    3205..3207
                     /label=Stop\codon
BASE COUNT     1748 a      2292 c      2066 g      1724 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca 
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa 
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac 
     2221 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 
     2281 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 
     2341 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 
     2401 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 
     2461 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 
     2521 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 
     2581 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 
     2641 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 
     2701 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 
     2761 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 
     2821 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 
     2881 ggcatggacg agctgtacaa gggatatcca tcacactggc ggccgctcga gcccagcccc 
     2941 gccaggcccg cactccgcgc cccggcctcc gctaccagtg gcagccgcaa gcgcgcccgc 
     3001 ccgcccgccg cccccggacg cgaccaggcc aggccaccgg cccgcaggag actgcggctg 
     3061 tcggtggacg aggtttccag ccccagtacc cccgaggccc cagacatccc agcctgccct 
     3121 tctccgggcc agaagataaa gaaatccacc ccggcagcag gtcagccgcc ccacctgaca 
     3181 tccgcgcagg accaggacac catctaactg atcataattc taccgggtag gggaggcgct 
     3241 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac 
     3301 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt 
     3361 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc 
     3421 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt 
     3481 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata 
     3541 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg 
     3601 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt 
     3661 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct 
     3721 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc 
     3781 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 
     3841 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 
     3901 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 
     3961 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 
     4021 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 
     4081 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 
     4141 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 
     4201 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 
     4261 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 
     4321 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg 
     4381 agcgcacgac cccatgcatc ggcgtctcga tcgagatatc agtggtccag gctctagttt 
     4441 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga 
     4501 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc 
     4561 tactagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga 
     4621 agttcagatc aaggtcagga acagatggaa cagggtcgat cgaccctaga gaaccatcag 
     4681 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat 
     4741 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca 
     4801 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat 
     4861 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct 
     4921 cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc 
     4981 gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg ctgcctcgcg 
     5041 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 
     5101 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 
     5161 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta gatccggctg 
     5221 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 
     5281 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 
     5341 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 
     5401 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 
     5461 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 
     5521 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttactggctt aactatgcgg 
     5581 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 
     5641 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     5701 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     5761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     5821 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     5881 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     5941 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     6001 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     6061 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     6121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     6181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     6241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     6301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     6361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     6421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     6481 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     6541 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     6601 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     6661 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     6721 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     6781 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     6841 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     6901 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     6961 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     7021 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     7081 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     7141 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     7201 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa 
     7261 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     7321 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     7381 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     7441 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     7501 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     7561 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 
     7621 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattag 
     7681 cttggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc 
     7741 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg 
     7801 ggtcattagt tcatagccca tatatggagt 
//
