LOCUS       pRetroQ-mCherry-N1   7597 bp    DNA   circular             7-OCT-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632568
FEATURES             Location/Qualifiers
     misc_feature    757..1566
                     /label=Extended\packaging\signal
     misc_feature    4296..4728
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     CDS             complement(6375..7235)
                     /label=Amp(R)
     rep_origin      complement(5615..5615)
                     /label=pUC\origin
     promoter        2983..3491
                     /label=PGK\Promoter
     CDS             3512..4111
                     /label=Puro(R)
     rep_origin      5229..5294
                     /label=SV40\origin
     misc_feature    2172..2246
                     /label=MCS
     promoter        5008..5275
                     /label=SV40\Promoter
     CDS             2254..2964
                     /label=mCherry
     CDS             1..728
                     /label=CMV/MSV\5'\LTR
     CDS             1582..2170
                     /label=CMV\IE\Promoter
BASE COUNT     1705 a      2178 c      2006 g      1708 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca 
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa 
     2161 ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag 
     2221 tcgacggtac cgcgggcccg ggatccaccg gtcatggtga gcaagggcga ggaggataac 
     2281 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 
     2341 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 
     2401 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 
     2461 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 
     2521 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 
     2581 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 
     2641 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 
     2701 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 
     2761 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 
     2821 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 
     2881 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 
     2941 ggcatggacg agctgtacaa gtgagcggcc gcgactctag ataattctac cgggtagggg 
     3001 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc 
     3061 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg 
     3121 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc 
     3181 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta 
     3241 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg 
     3301 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg 
     3361 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc 
     3421 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc 
     3481 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct 
     3541 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 
     3601 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 
     3661 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 
     3721 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 
     3781 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 
     3841 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 
     3901 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 
     3961 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 
     4021 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 
     4081 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac 
     4141 cgaaaggagc gcacgacccc atgcatcggc gtctcgatcg agatatcagt ggtccaggct 
     4201 ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa 
     4261 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt 
     4321 ggcaagctac tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga 
     4381 atagagaagt tcagatcaag gtcaggaaca gatggaacag ggtcgatcga ccctagagaa 
     4441 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact 
     4501 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa 
     4561 gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc 
     4621 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg 
     4681 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc 
     4741 cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg 
     4801 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 
     4861 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 
     4921 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtagat 
     4981 ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 
     5041 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 
     5101 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 
     5161 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     5221 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 
     5281 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta ctggcttaac 
     5341 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 
     5401 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 
     5461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     5521 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
     5581 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     5641 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     5701 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     5761 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
     5821 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     5881 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     5941 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     6001 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
     6061 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     6121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     6181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     6241 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     6301 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     6361 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     6421 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     6481 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     6541 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     6601 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     6661 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 
     6721 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     6781 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     6841 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     6901 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     6961 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga 
     7021 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     7081 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     7141 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     7201 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
     7261 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     7321 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 
     7381 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa 
     7441 gaattagctt ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc 
     7501 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca 
     7561 attacggggt cattagttca tagcccatat atggagt 
//
