LOCUS       pRetroQ-AcGFP1-N1   7606 bp    DNA   circular            10-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632505
FEATURES             Location/Qualifiers
     misc_feature    1..727
                     /label=5'\LTR\(CMV/MSV)
     misc_feature    757..1566
                     /label=Extended\packaging\signal
     misc_feature    4305..4737
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     CDS             complement(6384..7244)
                     /label=Amp(R)
     rep_origin      complement(5624..5624)
                     /label=pUC\origin
     promoter        2992..3500
                     /label=PGK\Promoter
     CDS             3521..4120
                     /label=Puro(R)
     rep_origin      5238..5303
                     /label=SV40\origin
     promoter        1582..2170
                     /label=CMV\IE\Promoter
                     /note="Human cytomegalovirus (CMV) immediate early promoter: Enhancer region: 59-465; TATA box: 554-560;Transcription start point: 583; C->G mutation to remove Sac I site: 569"
     CDS             2254..2970
                     /label=AcGFP1
     misc_feature    2172..2246
                     /label=MCS
     promoter        5017..5284
                     /label=SV40\Promoter
BASE COUNT     1715 a      2180 c      1983 g      1728 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca 
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa 
     2161 ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag 
     2221 tcgacggtac cgcgggcccg ggatccaccg gtcatggtga gcaagggcgc cgagctgttc 
     2281 accggcatcg tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc 
     2341 gtgagcggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 
     2401 accaccggca agctgcctgt gccctggccc accctggtga ccaccctgag ctacggcgtg 
     2461 cagtgcttct cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg 
     2521 cctgagggct acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagtcg 
     2581 cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc gcatcgagct gaccggcacc 
     2641 gatttcaagg aggatggcaa catcctgggc aataagatgg agtacaacta caacgcccac 
     2701 aatgtgtaca tcatgaccga caaggccaag aatggcatca aggtgaactt caagatccgc 
     2761 cacaacatcg aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc 
     2821 ggcgatggcc ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc 
     2881 aaggacccca acgagaagcg cgatcacatg atctacttcg gcttcgtgac cgccgccgcc 
     2941 atcacccacg gcatggatga gctgtacaag tgagcggccg cgactctaga taattctacc 
     3001 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg 
     3061 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc 
     3121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca 
     3181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac 
     3241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt 
     3301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg 
     3361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct 
     3421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt 
     3481 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac 
     3541 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 
     3601 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 
     3661 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 
     3721 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 
     3781 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 
     3841 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 
     3901 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 
     3961 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 
     4021 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 
     4081 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca 
     4141 gcgcccgacc gaaaggagcg cacgacccca tgcatcggcg tctcgatcga gatatcagtg 
     4201 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca 
     4261 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc 
     4321 tgtaggtttg gcaagctact agcttaagta acgccatttt gcaaggcatg gaaaaataca 
     4381 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagg gtcgatcgac 
     4441 cctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt 
     4501 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc 
     4561 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc 
     4621 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg 
     4681 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg 
     4741 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta 
     4801 agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 
     4861 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 
     4921 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 
     4981 agtgtagatc cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     5041 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     5101 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     5161 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     5221 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     5281 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac 
     5341 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 
     5401 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 
     5461 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     5521 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     5581 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     5641 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     5701 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     5761 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
     5821 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     5881 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     5941 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     6001 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     6061 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     6121 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     6181 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     6241 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     6301 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     6361 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     6421 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     6481 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     6541 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     6601 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     6661 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 
     6721 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     6781 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     6841 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     6901 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     6961 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 
     7021 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     7081 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     7141 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     7201 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     7261 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     7321 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     7381 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 
     7441 cgtcttcaag aattagcttg gccattgcat acgttgtatc catatcataa tatgtacatt 
     7501 tatattggct catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa 
     7561 tagtaatcaa ttacggggtc attagttcat agcccatata tggagt 
//
